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- W4313309944 abstract "Abstract In cultures of thymocytes or spleen cells from tetanus toxoid-primed mice, the addition of 1 ng of toxoid generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernatants were fractionated by gel filtration on Sephadex G-150 and Bio-Gel P-150, the active material eluted with molecules of approximately 75,000 m.w. Column fractions from similarly-treated supernatants from cultures of normal thymocytes or spleen cells, maintained with or without tetanus toxoid, were inactive. The enhancing activity recovered from the column fractions was degraded by treatment with the enzyme protease. A factor present in the supernatant fluid of cultures containing equal numbers of histoincompatible (allogeneic) spleen cells had similar properties. The enhancing factor was further purified by a combination of ammonium sulfate fractionation, Sephadex gel filtration, and polyacrylamide gel electrophoresis." @default.
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- W4313309944 date "1973-11-01" @default.
- W4313309944 modified "2023-09-27" @default.
- W4313309944 title "Specific Heterologous Enhancement of Immune Responses" @default.
- W4313309944 doi "https://doi.org/10.4049/jimmunol.111.5.1314" @default.
- W4313309944 hasPublicationYear "1973" @default.
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