Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313310007> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W4313310007 endingPage "763" @default.
- W4313310007 startingPage "756" @default.
- W4313310007 abstract "Abstract The evolution of the immune response to a bacterial antigen was studied in a closed in vitro system, whereby the physiologic architecture of the responding tissue is preserved and communications with central organs (thymus, bone marrow), circulating cell and antibody pools, and antigen depots are cut off. The time-course changes in binding properties of antibodies directed to a distinct determinant of native, wild type, Escherichia coli β-d-galactosidase were determined by measuring their association constant for a naturally occurring ligand, a defective point mutant enzyme (AMEF), at various intervals during the response of rabbit lymph node fragments, which had been challenged in vitro, washed, and placed in culture, individually or in groups of five. Early secondary antibodies were distributed over a wide range of affinities, although antibodies in the serum of the donor rabbit were restricted at the moment of sacrifice and initiation of cultures. Later on, a gradual increase in affinity with progressive restriction occurred more often in multifragment cultures and in cultures challenged with 50 µg/ml than in those challenged with 5 µg/ml of β-d-galactosidase. The magnitude of the affinity rise was inversely correlated with the association constant of early antibodies and the peak affinity reached in each culture did not go over the highest value recorded in the serum of the donor, with only few exceptions. This indicates that although there is a strong tendency to maturation, there is a certain ceiling which is rarely surpassed. In some cultures a late fall in K0 was observed together with increased antibody heterogeneity. The overall data indicate that during microevolution of the immune response in vitro against a natural determinant in its native molecular configuration, a progressive contraction of high-rate antibody-forming clones occurs toward high affinity. Expansion of the memory potential over a wide range of affinities takes place in parallel. The limits of these processes, top affinity reached, and width of memory spectrum seem to be mainly determined by a prefixed array of precursors or memory cells. However, generation of new affinities during the immune response also seems to be possible." @default.
- W4313310007 created "2023-01-06" @default.
- W4313310007 creator A5014042288 @default.
- W4313310007 creator A5087378104 @default.
- W4313310007 date "1974-09-01" @default.
- W4313310007 modified "2023-10-18" @default.
- W4313310007 title "Association Constant and Heterogeneity Index of Antibodies Specific for a Natural Determinant of a Bacterial Protein" @default.
- W4313310007 doi "https://doi.org/10.4049/jimmunol.113.3.756" @default.
- W4313310007 hasPublicationYear "1974" @default.
- W4313310007 type Work @default.
- W4313310007 citedByCount "1" @default.
- W4313310007 crossrefType "journal-article" @default.
- W4313310007 hasAuthorship W4313310007A5014042288 @default.
- W4313310007 hasAuthorship W4313310007A5087378104 @default.
- W4313310007 hasBestOaLocation W43133100071 @default.
- W4313310007 hasConcept C116569031 @default.
- W4313310007 hasConcept C147483822 @default.
- W4313310007 hasConcept C153911025 @default.
- W4313310007 hasConcept C159654299 @default.
- W4313310007 hasConcept C170493617 @default.
- W4313310007 hasConcept C181199279 @default.
- W4313310007 hasConcept C202751555 @default.
- W4313310007 hasConcept C203014093 @default.
- W4313310007 hasConcept C2780849966 @default.
- W4313310007 hasConcept C2781377902 @default.
- W4313310007 hasConcept C55493867 @default.
- W4313310007 hasConcept C86803240 @default.
- W4313310007 hasConcept C8891405 @default.
- W4313310007 hasConceptScore W4313310007C116569031 @default.
- W4313310007 hasConceptScore W4313310007C147483822 @default.
- W4313310007 hasConceptScore W4313310007C153911025 @default.
- W4313310007 hasConceptScore W4313310007C159654299 @default.
- W4313310007 hasConceptScore W4313310007C170493617 @default.
- W4313310007 hasConceptScore W4313310007C181199279 @default.
- W4313310007 hasConceptScore W4313310007C202751555 @default.
- W4313310007 hasConceptScore W4313310007C203014093 @default.
- W4313310007 hasConceptScore W4313310007C2780849966 @default.
- W4313310007 hasConceptScore W4313310007C2781377902 @default.
- W4313310007 hasConceptScore W4313310007C55493867 @default.
- W4313310007 hasConceptScore W4313310007C86803240 @default.
- W4313310007 hasConceptScore W4313310007C8891405 @default.
- W4313310007 hasIssue "3" @default.
- W4313310007 hasLocation W43133100071 @default.
- W4313310007 hasOpenAccess W4313310007 @default.
- W4313310007 hasPrimaryLocation W43133100071 @default.
- W4313310007 hasRelatedWork W1484614058 @default.
- W4313310007 hasRelatedWork W1484759019 @default.
- W4313310007 hasRelatedWork W1972479046 @default.
- W4313310007 hasRelatedWork W1986244198 @default.
- W4313310007 hasRelatedWork W2001055320 @default.
- W4313310007 hasRelatedWork W2149788084 @default.
- W4313310007 hasRelatedWork W2150135081 @default.
- W4313310007 hasRelatedWork W2162118286 @default.
- W4313310007 hasRelatedWork W2220102944 @default.
- W4313310007 hasRelatedWork W4313376859 @default.
- W4313310007 hasVolume "113" @default.
- W4313310007 isParatext "false" @default.
- W4313310007 isRetracted "false" @default.
- W4313310007 workType "article" @default.