Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313311255> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W4313311255 endingPage "268" @default.
- W4313311255 startingPage "259" @default.
- W4313311255 abstract "Abstract Immunoglobulin κ-chain mRNA was hybridized with DNA in order to assess the κ-gene frequency. κ-mRNA was purified from membrane-bound ribosomes of mouse myeloma MOPC-41 by poly (U) chromatography and isolation of a 13S RNA by successive sucrose density gradient centrifugations. The RNA coded for κ-chain precursor molecules in cell-free protein synthesis and essentially no other proteins. MOPC-41 κ-mRNA hybridized with MOPC-41, MPC-11, and Krebs DNA with the same kinetics: the majority of the hybrids was formed with rare or unique DNA sequences (Cot/2 450 to 900), a small portion with highly repetitive sequences (Cot/2 5–6). The slow hybrids were well matched and the rapid hybrids were mismatched by about 4%, regardless of the DNA used. It was further investigated whether the rapid hybrids contained translatable κ-mRNA or were due to impurities in the RNA preparations. κ-mRNA and globin-mRNA (as an internal standard for a unique transcript) were hybridized with DNA to Cot 20 or 48, the hybridized and unhybridized RNA were isolated by hydroxyopatite-urea chromatography and, after removal of the DNA, translated in a cell-free system. The cell-free products were analyzed by SDS-polyacrylamide gel electrophoresis and immunoprecipitation. It was found that approximately equal quantities of translatable κ- and globin-mRNA were hybridized maximally 1.7%). The results do not support the hypothesis that κ-mRNA is a transcript of both repetitive and unique DNA sequences." @default.
- W4313311255 created "2023-01-06" @default.
- W4313311255 creator A5006252115 @default.
- W4313311255 creator A5044065361 @default.
- W4313311255 date "1976-07-01" @default.
- W4313311255 modified "2023-10-16" @default.
- W4313311255 title "Analysis of Immunoglobulin Genes: DNA/RNA Hybridization with Immunoglobulin κ-Chain mRNA and Isolation and Translation of Hybridized RNA" @default.
- W4313311255 doi "https://doi.org/10.4049/jimmunol.117.1.259" @default.
- W4313311255 hasPublicationYear "1976" @default.
- W4313311255 type Work @default.
- W4313311255 citedByCount "1" @default.
- W4313311255 crossrefType "journal-article" @default.
- W4313311255 hasAuthorship W4313311255A5006252115 @default.
- W4313311255 hasAuthorship W4313311255A5044065361 @default.
- W4313311255 hasBestOaLocation W43133112551 @default.
- W4313311255 hasConcept C104317684 @default.
- W4313311255 hasConcept C105580179 @default.
- W4313311255 hasConcept C116084860 @default.
- W4313311255 hasConcept C150194340 @default.
- W4313311255 hasConcept C153911025 @default.
- W4313311255 hasConcept C54871578 @default.
- W4313311255 hasConcept C552990157 @default.
- W4313311255 hasConcept C55493867 @default.
- W4313311255 hasConcept C67705224 @default.
- W4313311255 hasConcept C86803240 @default.
- W4313311255 hasConcept C88478588 @default.
- W4313311255 hasConceptScore W4313311255C104317684 @default.
- W4313311255 hasConceptScore W4313311255C105580179 @default.
- W4313311255 hasConceptScore W4313311255C116084860 @default.
- W4313311255 hasConceptScore W4313311255C150194340 @default.
- W4313311255 hasConceptScore W4313311255C153911025 @default.
- W4313311255 hasConceptScore W4313311255C54871578 @default.
- W4313311255 hasConceptScore W4313311255C552990157 @default.
- W4313311255 hasConceptScore W4313311255C55493867 @default.
- W4313311255 hasConceptScore W4313311255C67705224 @default.
- W4313311255 hasConceptScore W4313311255C86803240 @default.
- W4313311255 hasConceptScore W4313311255C88478588 @default.
- W4313311255 hasIssue "1" @default.
- W4313311255 hasLocation W43133112551 @default.
- W4313311255 hasOpenAccess W4313311255 @default.
- W4313311255 hasPrimaryLocation W43133112551 @default.
- W4313311255 hasRelatedWork W1900224185 @default.
- W4313311255 hasRelatedWork W1977441654 @default.
- W4313311255 hasRelatedWork W1981662335 @default.
- W4313311255 hasRelatedWork W2002473487 @default.
- W4313311255 hasRelatedWork W2009966535 @default.
- W4313311255 hasRelatedWork W2037632559 @default.
- W4313311255 hasRelatedWork W2039244532 @default.
- W4313311255 hasRelatedWork W2064186857 @default.
- W4313311255 hasRelatedWork W2145879504 @default.
- W4313311255 hasRelatedWork W4313311255 @default.
- W4313311255 hasVolume "117" @default.
- W4313311255 isParatext "false" @default.
- W4313311255 isRetracted "false" @default.
- W4313311255 workType "article" @default.