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- W4313311562 abstract "Abstract Human B lymphocytes obtained from tonsils do not proliferate when stimulated with pokeweed mitogen. A soluble factor produced from T cells cultured with pokeweed mitogen stimulates B cells to synthesize DNA and differentiate into immunoglobulin producing cells. This PWM produced supernatant induced a PFC response to SRBC. The T cell supernatant activity is produced within 12 hr of stimulation in the presence of serum and without a requirement for T cell division. Optimal stimulation of B cells occurred at 7 to 9 days of culture. This helper factor activity eluted postalbumin from a column of Sephadex G-200. Insolubilized pokeweed mitogen was not mitogenic for B cells. The continuous presence of the lectin in culture was not required for B cell proliferation or for immunoglobulin synthesis." @default.
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- W4313311562 date "1977-06-01" @default.
- W4313311562 modified "2023-09-30" @default.
- W4313311562 title "The Necessity for T Cell Help for Human Tonsil B Cell Responses to Pokeweed Mitogen: Induction of DNA Synthesis, Immunoglobulin, and Specific Antibody Production with a T Cell Helper Factor Produced with Pokeweed Mitogen" @default.
- W4313311562 doi "https://doi.org/10.4049/jimmunol.118.6.2009" @default.
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