FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313314788> ?p ?o ?g. }

• W4313314788 abstract "ABSTRACT Japanese encephalitis virus (JEV) is a mosquito-borne Flavivirus , which may cause severe encephalitis in humans, horses, and other animals. TIM-1 has been identified to be a receptor that promotes various viruses to enter into target cells in recent years. In the present study, we found that TIM-1 protein was significantly increased in A549 cells at the late stage of JEV infection, while the transcription levels of TIM-1 remained unaltered. Interestingly, we found that NS1’ protein plays a key role in increasing the expression of TIM-1 in cells infected with JEV. Further, we found that the NS1’ protein also efficiently regulates TIM-1 protein to distribute in the cytoplasm in JEV-infected cells, and the amount of TIM-1 protein located on the cell membrane was reduced instead. As a consequence, NS1’ protein antagonize TIM-1 mediated viral restriction for further viral infection and propagation in the late stage of infection. In molecular mechanism, the molecular weight of TIM-1 increased a bit in the present of NS1’. Expression of NEU1 down-regulated TIM-1 expression and oseltamivir treatment increased the expression of TIM-1. Therefore, our data indicated that JEV NS1’ protein facilitated the sialylation modification of TIM-1 to up-regulate the level of TIM-1 expression and regulate its distribution. Collectively, our study revealed that JEV NS1’ protein regulates the expression and distribution of TIM-1 by facilitating its sialylation to antagonize TIM-1-mediated JEV restriction for further infection. Understanding the functional interplays between TIM-1 and NS1’ proteins will offer new insights into virushost interaction. IMPORTANCE T-cell immunoglobulin and mucin domain protein 1 (TIM-1) is known to promote cellular entry of various enveloped viruses. We discovered a novel phenomenon of dynamic expression and functional regulation of TIM-1 during JEV infection. Firstly, TIM-1 protein increased in the cytoplasm at the late stage of JEV infection. Furthermore, JEV NS1’ protein up-regulated the intracellular expression of TIM-1 and antagonize TIM-1-mediated viral restriction for further viral infection. In molecular mechanism, JEV NS1’ protein facilitated the sialylation of TIM-1 to increase the level of TIM-1 expression and cytoplasm distribution. Taken together, at the late stage of infection, JEV employs a strategy in which NS1’ promotes JEV release by promoting the sialylation of TIM-1, causing it to be primarily localized in the cytoplasm. Therefore, we discovered new functions of TIM-1 and JEV NS1’ during the process of JEV infection, and provide a new insight into the interactions between JEV and cell hosts." @default.
• W4313314788 created "2023-01-06" @default.
• W4313314788 creator A5007972336 @default.
• W4313314788 creator A5040234485 @default.
• W4313314788 creator A5044682495 @default.
• W4313314788 creator A5048948137 @default.
• W4313314788 creator A5071113427 @default.
• W4313314788 creator A5075546376 @default.
• W4313314788 date "2022-12-16" @default.
• W4313314788 modified "2023-10-14" @default.
• W4313314788 title "Japanese Encephalitis Virus NS1’ Protein regulates the expression and distribution of TIM-1 to promote viral infection" @default.
• W4313314788 cites W1966857012 @default.
• W4313314788 cites W1970948184 @default.
• W4313314788 cites W1972730346 @default.
• W4313314788 cites W1973879517 @default.
• W4313314788 cites W1975555235 @default.
• W4313314788 cites W2002545308 @default.
• W4313314788 cites W2005973706 @default.
• W4313314788 cites W2007610160 @default.
• W4313314788 cites W2037643815 @default.
• W4313314788 cites W2054624000 @default.
• W4313314788 cites W2071776498 @default.
• W4313314788 cites W2078998316 @default.
• W4313314788 cites W2080735658 @default.
• W4313314788 cites W2094655231 @default.
• W4313314788 cites W2122756910 @default.
• W4313314788 cites W2135004938 @default.
• W4313314788 cites W2138063446 @default.
• W4313314788 cites W2142577902 @default.
• W4313314788 cites W2145729467 @default.
• W4313314788 cites W2148548715 @default.
• W4313314788 cites W2166305421 @default.
• W4313314788 cites W2167048802 @default.
• W4313314788 cites W2167717479 @default.
• W4313314788 cites W2169112650 @default.
• W4313314788 cites W2169629544 @default.
• W4313314788 cites W2172179018 @default.
• W4313314788 cites W2187937073 @default.
• W4313314788 cites W2548008859 @default.
• W4313314788 cites W2579536201 @default.
• W4313314788 cites W26319236 @default.
• W4313314788 cites W2804966859 @default.
• W4313314788 cites W2901585414 @default.
• W4313314788 cites W2901894286 @default.
• W4313314788 cites W2903090136 @default.
• W4313314788 cites W2919901342 @default.
• W4313314788 cites W2947521426 @default.
• W4313314788 cites W2987686437 @default.
• W4313314788 cites W3003227744 @default.
• W4313314788 cites W3033053749 @default.
• W4313314788 cites W3047201386 @default.
• W4313314788 cites W3083628264 @default.
• W4313314788 cites W3186235333 @default.
• W4313314788 cites W3198617403 @default.
• W4313314788 cites W3211841736 @default.
• W4313314788 cites W4224986719 @default.
• W4313314788 cites W4226035580 @default.
• W4313314788 cites W95293157 @default.
• W4313314788 doi "https://doi.org/10.1101/2022.12.15.520687" @default.
• W4313314788 hasPublicationYear "2022" @default.
• W4313314788 type Work @default.
• W4313314788 citedByCount "0" @default.
• W4313314788 crossrefType "posted-content" @default.
• W4313314788 hasAuthorship W4313314788A5007972336 @default.
• W4313314788 hasAuthorship W4313314788A5040234485 @default.
• W4313314788 hasAuthorship W4313314788A5044682495 @default.
• W4313314788 hasAuthorship W4313314788A5048948137 @default.
• W4313314788 hasAuthorship W4313314788A5071113427 @default.
• W4313314788 hasAuthorship W4313314788A5075546376 @default.
• W4313314788 hasBestOaLocation W43133147881 @default.
• W4313314788 hasConcept C159047783 @default.
• W4313314788 hasConcept C190062978 @default.
• W4313314788 hasConcept C2522874641 @default.
• W4313314788 hasConcept C2779141303 @default.
• W4313314788 hasConcept C2779734145 @default.
• W4313314788 hasConcept C2780568436 @default.
• W4313314788 hasConcept C83455156 @default.
• W4313314788 hasConcept C86803240 @default.
• W4313314788 hasConcept C95444343 @default.
• W4313314788 hasConceptScore W4313314788C159047783 @default.
• W4313314788 hasConceptScore W4313314788C190062978 @default.
• W4313314788 hasConceptScore W4313314788C2522874641 @default.
• W4313314788 hasConceptScore W4313314788C2779141303 @default.
• W4313314788 hasConceptScore W4313314788C2779734145 @default.
• W4313314788 hasConceptScore W4313314788C2780568436 @default.
• W4313314788 hasConceptScore W4313314788C83455156 @default.
• W4313314788 hasConceptScore W4313314788C86803240 @default.
• W4313314788 hasConceptScore W4313314788C95444343 @default.
• W4313314788 hasLocation W43133147881 @default.
• W4313314788 hasOpenAccess W4313314788 @default.
• W4313314788 hasPrimaryLocation W43133147881 @default.
• W4313314788 hasRelatedWork W1977243449 @default.
• W4313314788 hasRelatedWork W2042506043 @default.
• W4313314788 hasRelatedWork W2057550814 @default.
• W4313314788 hasRelatedWork W2144567501 @default.
• W4313314788 hasRelatedWork W2167490505 @default.
• W4313314788 hasRelatedWork W2169820084 @default.
• W4313314788 hasRelatedWork W2188759708 @default.
• W4313314788 hasRelatedWork W2791292804 @default.
• W4313314788 hasRelatedWork W2885999504 @default.

• next