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• W4313331908 abstract "Three-dimensional (3D) imaging and object tracking is critical for medical and biological research and can be achieved by multifocal imaging with diffractive optical elements (DOEs) converting depth ( z ) information into a modification of the two-dimensional image. Physical insight into DOE designs will spur this expanding field.To precisely track microscopic fluorescent objects in biological systems in 3D with a simple low-cost DOE system.We designed a multiring spiral phase plate (SPP) generating a single-spot rotating point spread function (SS-RPSF) in a microscope. Our simple, analytically transparent design process uses Bessel beams to avoid rotational ambiguities and achieve a significant depth range. The SPP was inserted into the Nomarski prism slider of a standard microscope. Performance was evaluated using fluorescent beads and in live cells expressing a fluorescent chromatin marker.Bead localization precision was <25 nm in the transverse dimensions and ≤70 nm along the axial dimension over an axial range of 6 μm . Higher axial precision ( ≤50 nm ) was achieved over a shallower focal depth of 2.9 μm . 3D diffusion constants of chromatin matched expected values.Precise 3D localization and tracking can be achieved with a SS-RPSF SPP in a standard microscope with minor modifications." @default.
• W4313331908 created "2023-01-06" @default.
• W4313331908 creator A5022603797 @default.
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• W4313331908 date "2022-12-29" @default.
• W4313331908 modified "2023-10-05" @default.
• W4313331908 title "Three-dimensional tracking using a single-spot rotating point spread function created by a multiring spiral phase plate" @default.
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• W4313331908 doi "https://doi.org/10.1117/1.jbo.27.12.126501" @default.
• W4313331908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36590978" @default.
• W4313331908 hasPublicationYear "2022" @default.
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