Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313333694> ?p ?o ?g. }
- W4313333694 endingPage "5069" @default.
- W4313333694 startingPage "5061" @default.
- W4313333694 abstract "Abstract Access of intracellular Ags SSA/Ro and SSB/La to cognate maternal autoantibodies is unexplained despite their strong association with congenital heart block. To investigate the hypothesis that apoptosis facilitates surface accessibility of these Ags, human fetal cardiac myocytes from 16- to 22-wk abortuses were established in culture using a novel technique in which cells were isolated after perfusing the aorta with collagenase. Confirmation of cardiac myocytes included positive staining with antisarcomeric α-actinin and contractility induced by 1.8 mM calcium. Incubation with 0.5 μM staurosporine or 0.3 mM 2,3-dimethoxy-1,4-naphthoquinone induced the characteristic morphologic and biochemical changes of apoptosis. The cellular topology of Ro and La was evaluated with confocal microscopy and determined in nonapoptotic and apoptotic cardiocytes by indirect immunofluorescence. In permeabilized nonapoptotic cardiocytes, Ro and La were predominantly nuclear, and propidium iodide (PI) stained the nucleus. In early apoptotic cardiocytes, condensation of the PI- and Ro- or La-stained nucleus was observed, accompanied by Ro/La fluorescence around the cell periphery. In later stages of apoptosis, nuclear Ro and La staining became weaker, and PI demonstrated nuclear fragmentation. Ro/La-stained blebs emerged from the cell membrane, a finding observed in nonpermeabilized cells, supporting an Ab-Ag interaction at the cell surface. In summary, induction of apoptosis in cultured cardiocytes results in surface translocation of Ro/La and recognition by Abs. Although apoptotic cells are programmed to die and do not characteristically evoke inflammation, binding of maternal Abs and subsequent influx of leukocytes could damage surrounding healthy fetal cardiocytes." @default.
- W4313333694 created "2023-01-06" @default.
- W4313333694 creator A5015631803 @default.
- W4313333694 creator A5019630657 @default.
- W4313333694 creator A5041023019 @default.
- W4313333694 creator A5042450848 @default.
- W4313333694 creator A5053140449 @default.
- W4313333694 creator A5063197768 @default.
- W4313333694 creator A5072473587 @default.
- W4313333694 creator A5075058978 @default.
- W4313333694 date "1998-11-01" @default.
- W4313333694 modified "2023-10-16" @default.
- W4313333694 title "Accessibility of SSA/Ro and SSB/La Antigens to Maternal Autoantibodies in Apoptotic Human Fetal Cardiac Myocytes" @default.
- W4313333694 cites W1229799089 @default.
- W4313333694 cites W1519733404 @default.
- W4313333694 cites W1544998723 @default.
- W4313333694 cites W1571251056 @default.
- W4313333694 cites W1582424095 @default.
- W4313333694 cites W1671694443 @default.
- W4313333694 cites W1967128858 @default.
- W4313333694 cites W1969108300 @default.
- W4313333694 cites W1971577556 @default.
- W4313333694 cites W1973141899 @default.
- W4313333694 cites W1977798629 @default.
- W4313333694 cites W1978023215 @default.
- W4313333694 cites W1990944487 @default.
- W4313333694 cites W1993830297 @default.
- W4313333694 cites W1994466512 @default.
- W4313333694 cites W1995266188 @default.
- W4313333694 cites W1997481325 @default.
- W4313333694 cites W1997807415 @default.
- W4313333694 cites W1998401262 @default.
- W4313333694 cites W2000293147 @default.
- W4313333694 cites W2002205128 @default.
- W4313333694 cites W2002963487 @default.
- W4313333694 cites W2003920739 @default.
- W4313333694 cites W2006874915 @default.
- W4313333694 cites W2006964904 @default.
- W4313333694 cites W2009315849 @default.
- W4313333694 cites W2009649067 @default.
- W4313333694 cites W2011283239 @default.
- W4313333694 cites W2017344650 @default.
- W4313333694 cites W2027304696 @default.
- W4313333694 cites W2028111124 @default.
- W4313333694 cites W2033011464 @default.
- W4313333694 cites W2034975699 @default.
- W4313333694 cites W2045850215 @default.
- W4313333694 cites W2046572926 @default.
- W4313333694 cites W2054157077 @default.
- W4313333694 cites W2057816765 @default.
- W4313333694 cites W2060658172 @default.
- W4313333694 cites W2062687834 @default.
- W4313333694 cites W2073090429 @default.
- W4313333694 cites W2074711374 @default.
- W4313333694 cites W2075831717 @default.
- W4313333694 cites W2077079333 @default.
- W4313333694 cites W2077248019 @default.
- W4313333694 cites W2079355126 @default.
- W4313333694 cites W2081131191 @default.
- W4313333694 cites W2082076638 @default.
- W4313333694 cites W2084125668 @default.
- W4313333694 cites W2089578769 @default.
- W4313333694 cites W2090603099 @default.
- W4313333694 cites W2099707359 @default.
- W4313333694 cites W210302837 @default.
- W4313333694 cites W2112321962 @default.
- W4313333694 cites W2114148064 @default.
- W4313333694 cites W2115561370 @default.
- W4313333694 cites W2116183231 @default.
- W4313333694 cites W2120751716 @default.
- W4313333694 cites W2131274358 @default.
- W4313333694 cites W2136172467 @default.
- W4313333694 cites W2138004203 @default.
- W4313333694 cites W2140582776 @default.
- W4313333694 cites W2150840069 @default.
- W4313333694 cites W2163841316 @default.
- W4313333694 cites W2168802481 @default.
- W4313333694 cites W2169470548 @default.
- W4313333694 cites W2171526567 @default.
- W4313333694 cites W2260292839 @default.
- W4313333694 cites W2321533542 @default.
- W4313333694 cites W2327871226 @default.
- W4313333694 cites W2329744871 @default.
- W4313333694 cites W2335088247 @default.
- W4313333694 cites W2412536772 @default.
- W4313333694 cites W2751552992 @default.
- W4313333694 cites W39614084 @default.
- W4313333694 cites W73108703 @default.
- W4313333694 doi "https://doi.org/10.4049/jimmunol.161.9.5061" @default.
- W4313333694 hasPublicationYear "1998" @default.
- W4313333694 type Work @default.
- W4313333694 citedByCount "73" @default.
- W4313333694 countsByYear W43133336942012 @default.
- W4313333694 countsByYear W43133336942013 @default.
- W4313333694 countsByYear W43133336942014 @default.
- W4313333694 countsByYear W43133336942015 @default.
- W4313333694 countsByYear W43133336942016 @default.
- W4313333694 countsByYear W43133336942017 @default.