Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313333816> ?p ?o ?g. }
- W4313333816 endingPage "2300" @default.
- W4313333816 startingPage "2290" @default.
- W4313333816 abstract "Abstract The mouse has approximately 140 germline Vκ genes, and functional Vκ exons are expressed at roughly equivalent levels in the preimmune repertoire. We have examined the expression of individual members of the Vκ10 family. Vκ10A and Vκ10B genes have been utilized in numerous hybridomas and myelomas, while Vκ10C has not. In this study, we have cloned the Vκ10C gene and shown that it is structurally functional, has the expected promoter elements and recombination signal sequences, and that it is capable of recombination. Vκ10C mRNA, however, is present at levels at least 1000-fold lower than Vκ10A and Vκ10B in adult spleens. While there are no sequence differences in the octamer or TATA box between Vκ10C and Vκ10A, there are three nucleotide changes in the promoter region. These promoters equally drive the expression of a reporter gene in B cells or plasma cells, but the Vκ10A promoter is able to drive expression in pre-B cell lines significantly better than the Vκ10C promoter (p < 0.05). Vκ10C rearrangements can be detected in bone marrow and splenic DNA. Therefore, the lack of Vκ10C expression may reflect the inability of Vκ10C-rearranged cells to undergo positive or negative selection. Our results suggest that the available Ab repertoire is shaped not only by the number of structurally functional genes, but also by the ability of assembled genes to be expressed at critical points during B cell maturation." @default.
- W4313333816 created "2023-01-06" @default.
- W4313333816 creator A5009698622 @default.
- W4313333816 creator A5026618493 @default.
- W4313333816 creator A5053972086 @default.
- W4313333816 date "1998-09-01" @default.
- W4313333816 modified "2023-09-26" @default.
- W4313333816 title "Asymmetric Contribution to Ig Repertoire Diversity by Vκ Exons: Differences in the Utilization of Vκ10 Exons" @default.
- W4313333816 cites W1472803246 @default.
- W4313333816 cites W1482163480 @default.
- W4313333816 cites W1497992740 @default.
- W4313333816 cites W1501075747 @default.
- W4313333816 cites W1515872856 @default.
- W4313333816 cites W1518461523 @default.
- W4313333816 cites W1534269392 @default.
- W4313333816 cites W1545458002 @default.
- W4313333816 cites W1552645156 @default.
- W4313333816 cites W1553946161 @default.
- W4313333816 cites W1565074690 @default.
- W4313333816 cites W1601749249 @default.
- W4313333816 cites W1638779458 @default.
- W4313333816 cites W1642478430 @default.
- W4313333816 cites W1909963133 @default.
- W4313333816 cites W1943538236 @default.
- W4313333816 cites W1961705126 @default.
- W4313333816 cites W1964459498 @default.
- W4313333816 cites W1964625649 @default.
- W4313333816 cites W1966254031 @default.
- W4313333816 cites W1968496468 @default.
- W4313333816 cites W1975868741 @default.
- W4313333816 cites W1979467809 @default.
- W4313333816 cites W1981102229 @default.
- W4313333816 cites W1986477747 @default.
- W4313333816 cites W1992730412 @default.
- W4313333816 cites W1996711595 @default.
- W4313333816 cites W1999759851 @default.
- W4313333816 cites W2001187632 @default.
- W4313333816 cites W2002499610 @default.
- W4313333816 cites W2010440546 @default.
- W4313333816 cites W2011859878 @default.
- W4313333816 cites W2013545053 @default.
- W4313333816 cites W2016850454 @default.
- W4313333816 cites W2020035310 @default.
- W4313333816 cites W2022805735 @default.
- W4313333816 cites W2024112963 @default.
- W4313333816 cites W2024208188 @default.
- W4313333816 cites W2026369483 @default.
- W4313333816 cites W2027255721 @default.
- W4313333816 cites W2039019845 @default.
- W4313333816 cites W2048795531 @default.
- W4313333816 cites W2051823651 @default.
- W4313333816 cites W2054488343 @default.
- W4313333816 cites W2058660996 @default.
- W4313333816 cites W2062028511 @default.
- W4313333816 cites W2063311322 @default.
- W4313333816 cites W2064262827 @default.
- W4313333816 cites W2064498617 @default.
- W4313333816 cites W2067845200 @default.
- W4313333816 cites W2077640739 @default.
- W4313333816 cites W2078428042 @default.
- W4313333816 cites W2080160583 @default.
- W4313333816 cites W2080376481 @default.
- W4313333816 cites W2086252935 @default.
- W4313333816 cites W2087668204 @default.
- W4313333816 cites W2089184579 @default.
- W4313333816 cites W2090867705 @default.
- W4313333816 cites W2092415281 @default.
- W4313333816 cites W2093638047 @default.
- W4313333816 cites W2109298936 @default.
- W4313333816 cites W2120513063 @default.
- W4313333816 cites W2125622789 @default.
- W4313333816 cites W2133061080 @default.
- W4313333816 cites W2152887701 @default.
- W4313333816 cites W2153701918 @default.
- W4313333816 cites W2165809537 @default.
- W4313333816 cites W2186603387 @default.
- W4313333816 cites W3151184720 @default.
- W4313333816 cites W4245688132 @default.
- W4313333816 cites W4252882061 @default.
- W4313333816 doi "https://doi.org/10.4049/jimmunol.161.5.2290" @default.
- W4313333816 hasPublicationYear "1998" @default.
- W4313333816 type Work @default.
- W4313333816 citedByCount "4" @default.
- W4313333816 countsByYear W43133338162014 @default.
- W4313333816 crossrefType "journal-article" @default.
- W4313333816 hasAuthorship W4313333816A5009698622 @default.
- W4313333816 hasAuthorship W4313333816A5026618493 @default.
- W4313333816 hasAuthorship W4313333816A5053972086 @default.
- W4313333816 hasBestOaLocation W43133338161 @default.
- W4313333816 hasConcept C101762097 @default.
- W4313333816 hasConcept C104317684 @default.
- W4313333816 hasConcept C109825262 @default.
- W4313333816 hasConcept C113646796 @default.
- W4313333816 hasConcept C121332964 @default.
- W4313333816 hasConcept C150194340 @default.
- W4313333816 hasConcept C153911025 @default.
- W4313333816 hasConcept C156695909 @default.
- W4313333816 hasConcept C157402786 @default.