FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313334051> ?p ?o ?g. }

• W4313334051 endingPage "102851" @default.
• W4313334051 startingPage "102851" @default.
• W4313334051 abstract "Misfolded proteins are recognized and degraded through protein quality control (PQC) pathways, which are essential for maintaining proteostasis and normal cellular functions. Defects in PQC can result in disease, including cancer, cardiovascular disease, and neurodegeneration. The small ubiquitin-related modifiers (SUMOs) were previously implicated in the degradation of nuclear misfolded proteins, but their functions in cytoplasmic PQC are unclear. Here, in a systematic screen of SUMO protein mutations in the budding yeast Saccharomyces cerevisiae, we identified a mutant allele (Smt3-K38A/K40A) that sensitizes cells to proteotoxic stress induced by amino acid analogs. Smt3-K38A/K40A mutant strains also exhibited a defect in the turnover of a soluble PQC model substrate containing the CL1 degron (NES-GFP-Ura3-CL1) localized in the cytoplasm, but not the nucleus. Using human U2OS SUMO1- and SUMO2-KO cell lines, we observed a similar SUMO-dependent pathway for degradation of the mammalian degron-containing PQC reporter protein, GFP-CL1, also only in the cytoplasm but not the nucleus. Moreover, we found that turnover of GFP-CL1 in the cytoplasm was uniquely dependent on SUMO1 but not the SUMO2 paralogue. Additionally, we showed that turnover of GFP-CL1 in the cytoplasm is dependent on the AAA-ATPase, Cdc48/p97. Cellular fractionation studies and analysis of a SUMO1-GFP-CL1 fusion protein revealed that SUMO1 promotes cytoplasmic misfolded protein degradation by maintaining substrate solubility. Collectively, our findings reveal a conserved and previously unrecognized role for SUMO1 in regulating cytoplasmic PQC and provide valuable insights into the roles of sumoylation in PQC-associated diseases." @default.
• W4313334051 created "2023-01-06" @default.
• W4313334051 creator A5018251715 @default.
• W4313334051 creator A5030131037 @default.
• W4313334051 creator A5036040264 @default.
• W4313334051 creator A5046597133 @default.
• W4313334051 creator A5061181377 @default.
• W4313334051 creator A5062443410 @default.
• W4313334051 date "2023-02-01" @default.
• W4313334051 modified "2023-10-01" @default.
• W4313334051 title "Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control" @default.
• W4313334051 cites W1490806999 @default.
• W4313334051 cites W1503691982 @default.
• W4313334051 cites W1579340161 @default.
• W4313334051 cites W1963488988 @default.
• W4313334051 cites W1965249601 @default.
• W4313334051 cites W1974256456 @default.
• W4313334051 cites W1975059674 @default.
• W4313334051 cites W1997369314 @default.
• W4313334051 cites W2014231458 @default.
• W4313334051 cites W2032612990 @default.
• W4313334051 cites W2043927273 @default.
• W4313334051 cites W2044287615 @default.
• W4313334051 cites W2047166991 @default.
• W4313334051 cites W2048161143 @default.
• W4313334051 cites W2048973024 @default.
• W4313334051 cites W2050895547 @default.
• W4313334051 cites W2057294377 @default.
• W4313334051 cites W2060168110 @default.
• W4313334051 cites W2061774656 @default.
• W4313334051 cites W2072022791 @default.
• W4313334051 cites W2076774576 @default.
• W4313334051 cites W2084666034 @default.
• W4313334051 cites W2086660631 @default.
• W4313334051 cites W2093520089 @default.
• W4313334051 cites W2094557361 @default.
• W4313334051 cites W2100200896 @default.
• W4313334051 cites W2100929124 @default.
• W4313334051 cites W2103074028 @default.
• W4313334051 cites W2106071043 @default.
• W4313334051 cites W2112441454 @default.
• W4313334051 cites W2122500226 @default.
• W4313334051 cites W2128719376 @default.
• W4313334051 cites W2135711463 @default.
• W4313334051 cites W2137755719 @default.
• W4313334051 cites W2139180375 @default.
• W4313334051 cites W2139188448 @default.
• W4313334051 cites W2145922063 @default.
• W4313334051 cites W2148947476 @default.
• W4313334051 cites W2154492443 @default.
• W4313334051 cites W2155808639 @default.
• W4313334051 cites W2156374607 @default.
• W4313334051 cites W2157360354 @default.
• W4313334051 cites W2157388608 @default.
• W4313334051 cites W2158270932 @default.
• W4313334051 cites W2160355176 @default.
• W4313334051 cites W2163056216 @default.
• W4313334051 cites W2167279371 @default.
• W4313334051 cites W2304280967 @default.
• W4313334051 cites W2320634483 @default.
• W4313334051 cites W2321509365 @default.
• W4313334051 cites W2350919937 @default.
• W4313334051 cites W2392382529 @default.
• W4313334051 cites W2462325035 @default.
• W4313334051 cites W2522044975 @default.
• W4313334051 cites W2587750756 @default.
• W4313334051 cites W2588138461 @default.
• W4313334051 cites W2605285953 @default.
• W4313334051 cites W2606154134 @default.
• W4313334051 cites W2769061006 @default.
• W4313334051 cites W2794441819 @default.
• W4313334051 cites W2801346090 @default.
• W4313334051 cites W2907177719 @default.
• W4313334051 cites W2948775193 @default.
• W4313334051 cites W2968185949 @default.
• W4313334051 cites W2969986880 @default.
• W4313334051 cites W2976701158 @default.
• W4313334051 cites W3089452157 @default.
• W4313334051 cites W3120386634 @default.
• W4313334051 cites W3179146692 @default.
• W4313334051 cites W3181404929 @default.
• W4313334051 cites W3207815989 @default.
• W4313334051 cites W4206081698 @default.
• W4313334051 cites W4210429133 @default.
• W4313334051 cites W4214562979 @default.
• W4313334051 cites W4214578575 @default.
• W4313334051 cites W4283377063 @default.
• W4313334051 cites W2805835720 @default.
• W4313334051 doi "https://doi.org/10.1016/j.jbc.2022.102851" @default.
• W4313334051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36587767" @default.
• W4313334051 hasPublicationYear "2023" @default.
• W4313334051 type Work @default.
• W4313334051 citedByCount "1" @default.
• W4313334051 countsByYear W43133340512023 @default.
• W4313334051 crossrefType "journal-article" @default.
• W4313334051 hasAuthorship W4313334051A5018251715 @default.
• W4313334051 hasAuthorship W4313334051A5030131037 @default.
• W4313334051 hasAuthorship W4313334051A5036040264 @default.

• next