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- W4313336254 abstract "Avocado fruit contains a substantial amount of triglycerides (TGs) along with a high content of unsaturated fatty acids. It is also rich in many other bioactive phytochemicals such as carotenoids, tocopherols, phytosterols, aliphatic alcohols, and hydrocarbons. This study was conducted during 2021 at the genetic lab, University of Limpopo, South Africa to compare two different extraction DNA methods from avocado. The DNA from the avocado was extracted using Chelex method 1 (overnight incubation at 56°C) and method 2 (Ten-minute incubation at 95°C) from the fresh leaves of the avocado. The quantitative and qualitative parameters were measured using a spectrophotometer. PCR reaction with primers for 28S were used on all samples to confirm and evaluate the extracted DNA. The results showed that the highest quality of extracted DNA was in method 2 (1.54-1.68). The quantitative and qualitative tests for PCR reaction showed that the DNA extracted using method 2 had better quality of DNA than method 1. In conclusion, both methods worked, but method two showed better results." @default.
- W4313336254 created "2023-01-06" @default.
- W4313336254 creator A5064084832 @default.
- W4313336254 date "2022-12-17" @default.
- W4313336254 modified "2023-09-30" @default.
- W4313336254 title "Comparison of two methods for DNA extraction from Avocado (Persea americana)" @default.
- W4313336254 doi "https://doi.org/10.31830/2348-7542.2022.roc-851" @default.
- W4313336254 hasPublicationYear "2022" @default.
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