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• W4313339872 abstract "Neurotensin (NT) is a 13-residue endogenous peptide found in mammals, with neurotransmission and hormonal roles in the central nervous system and gastrointestinal tract, respectively. The first residue of NT is a pyroglutamate (pGlu) that makes the expression and purification of large amounts of NT with native modification challenging. Here, we describe a simple and efficient procedure for expression and purification of large amounts of NT based on using the small ubiquitin-like modifier (SUMO) as a fusion partner and subsequent enzymatic conversion of the N-terminal glutamine to pGlu. Yields of 13 mg/L and 8 mg/L of pure peptide were obtained from expression in rich and minimal media, respectively. The method is adaptable to expression and purification of proteins and peptides with pGlu modification in a wide range of eukaryotic and prokaryotic expression hosts." @default.
• W4313339872 created "2023-01-06" @default.
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• W4313339872 date "2023-04-01" @default.
• W4313339872 modified "2023-10-18" @default.
• W4313339872 title "High yield expression and purification of full-length Neurotensin with pyroglutamate modification" @default.
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• W4313339872 doi "https://doi.org/10.1016/j.pep.2022.106227" @default.
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