Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313339937> ?p ?o ?g. }
- W4313339937 endingPage "1793" @default.
- W4313339937 startingPage "1773" @default.
- W4313339937 abstract "Transcriptomic analysis is a powerful method in the utilization of New Approach Methods (NAMs) for identifying mechanisms of toxicity and application to hazard characterization. With this regard, mapping toxicological events to time of exposure would be helpful to characterize early events. Here, we investigated time-dependent changes in gene expression levels in iPSC-derived renal proximal tubular-like cells (PTL) treated with five diverse compounds using TempO-Seq transcriptomics with the aims to evaluate the application of PTL for toxicity prediction and to report on temporal effects for the activation of cellular stress response pathways. PTL were treated with either 50 μM amiodarone, 10 μM sodium arsenate, 5 nM rotenone, or 300 nM tunicamycin over a temporal time course between 1 and 24 h. The TGFβ-type I receptor kinase inhibitor GW788388 (1 μM) was used as a negative control. Pathway analysis revealed the induction of key stress-response pathways, including Nrf2 oxidative stress response, unfolding protein response, and metal stress response. Early response genes per pathway were identified much earlier than 24 h and included HMOX1, ATF3, DDIT3, and several MT1 isotypes. GW788388 did not induce any genes within the stress response pathways above, but showed deregulation of genes involved in TGFβ inhibition, including downregulation of CYP24A1 and SERPINE1 and upregulation of WT1. This study highlights the application of iPSC-derived renal cells for prediction of cellular toxicity and sheds new light on the temporal and early effects of key genes that are involved in cellular stress response pathways." @default.
- W4313339937 created "2023-01-06" @default.
- W4313339937 creator A5018032298 @default.
- W4313339937 creator A5021308779 @default.
- W4313339937 creator A5027572512 @default.
- W4313339937 creator A5028987042 @default.
- W4313339937 creator A5036088411 @default.
- W4313339937 creator A5037308317 @default.
- W4313339937 creator A5056483916 @default.
- W4313339937 creator A5090623663 @default.
- W4313339937 date "2022-12-31" @default.
- W4313339937 modified "2023-10-14" @default.
- W4313339937 title "Capturing time-dependent activation of genes and stress-response pathways using transcriptomics in iPSC-derived renal proximal tubule cells" @default.
- W4313339937 cites W1967067593 @default.
- W4313339937 cites W1972755859 @default.
- W4313339937 cites W1975416756 @default.
- W4313339937 cites W1980913432 @default.
- W4313339937 cites W1989277387 @default.
- W4313339937 cites W1990421784 @default.
- W4313339937 cites W1993726528 @default.
- W4313339937 cites W1994417313 @default.
- W4313339937 cites W1996046117 @default.
- W4313339937 cites W1998973923 @default.
- W4313339937 cites W1999758530 @default.
- W4313339937 cites W2011835391 @default.
- W4313339937 cites W2012563646 @default.
- W4313339937 cites W2013051865 @default.
- W4313339937 cites W2041880009 @default.
- W4313339937 cites W2047224011 @default.
- W4313339937 cites W2047244443 @default.
- W4313339937 cites W2059224128 @default.
- W4313339937 cites W2059834034 @default.
- W4313339937 cites W2066609845 @default.
- W4313339937 cites W2067299488 @default.
- W4313339937 cites W2073400184 @default.
- W4313339937 cites W2087413420 @default.
- W4313339937 cites W2089886418 @default.
- W4313339937 cites W2091705039 @default.
- W4313339937 cites W2122345241 @default.
- W4313339937 cites W2139339913 @default.
- W4313339937 cites W2144072633 @default.
- W4313339937 cites W2150420891 @default.
- W4313339937 cites W2162143298 @default.
- W4313339937 cites W2179438025 @default.
- W4313339937 cites W2206971521 @default.
- W4313339937 cites W2782405693 @default.
- W4313339937 cites W2808204293 @default.
- W4313339937 cites W2876121811 @default.
- W4313339937 cites W2884738449 @default.
- W4313339937 cites W2895325788 @default.
- W4313339937 cites W2897133367 @default.
- W4313339937 cites W2899007325 @default.
- W4313339937 cites W2949051248 @default.
- W4313339937 cites W2951839125 @default.
- W4313339937 cites W2983299232 @default.
- W4313339937 cites W2986671954 @default.
- W4313339937 cites W2990824114 @default.
- W4313339937 cites W3004147540 @default.
- W4313339937 cites W3017740089 @default.
- W4313339937 cites W3046087118 @default.
- W4313339937 cites W3083058234 @default.
- W4313339937 cites W3096051024 @default.
- W4313339937 cites W3113197893 @default.
- W4313339937 cites W3119583264 @default.
- W4313339937 cites W3121083171 @default.
- W4313339937 cites W3123047942 @default.
- W4313339937 cites W3126918553 @default.
- W4313339937 cites W3164118628 @default.
- W4313339937 cites W3191327805 @default.
- W4313339937 cites W3196767092 @default.
- W4313339937 cites W3217632966 @default.
- W4313339937 cites W4200117647 @default.
- W4313339937 cites W4213267533 @default.
- W4313339937 cites W4221034812 @default.
- W4313339937 cites W4223601057 @default.
- W4313339937 cites W4231254854 @default.
- W4313339937 doi "https://doi.org/10.1007/s10565-022-09783-5" @default.
- W4313339937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36586010" @default.
- W4313339937 hasPublicationYear "2022" @default.
- W4313339937 type Work @default.
- W4313339937 citedByCount "0" @default.
- W4313339937 crossrefType "journal-article" @default.
- W4313339937 hasAuthorship W4313339937A5018032298 @default.
- W4313339937 hasAuthorship W4313339937A5021308779 @default.
- W4313339937 hasAuthorship W4313339937A5027572512 @default.
- W4313339937 hasAuthorship W4313339937A5028987042 @default.
- W4313339937 hasAuthorship W4313339937A5036088411 @default.
- W4313339937 hasAuthorship W4313339937A5037308317 @default.
- W4313339937 hasAuthorship W4313339937A5056483916 @default.
- W4313339937 hasAuthorship W4313339937A5090623663 @default.
- W4313339937 hasBestOaLocation W43133399371 @default.
- W4313339937 hasConcept C104317684 @default.
- W4313339937 hasConcept C127561419 @default.
- W4313339937 hasConcept C150194340 @default.
- W4313339937 hasConcept C162317418 @default.
- W4313339937 hasConcept C2776151105 @default.
- W4313339937 hasConcept C2780096491 @default.
- W4313339937 hasConcept C54355233 @default.