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- W4313347815 abstract "Abstract The ZAP70/Syk family of protein tyrosine kinases plays an important role in Ag receptor signaling. Structural similarity of Syk and ZAP70 suggests their functional overlap. Previously, it was observed that expression of either ZAP70 or Syk reconstitutes Ag receptor signaling in Syk-negative B cells. However, in CD45-deficient T cells, Syk, but not ZAP70, restores T cell receptor-signaling pathway. To study the function of Syk, ZAP70, and CD45 in mast cells, a Syk/CD45 double-deficient variant of RBL-2H3 cells was characterized. After transfection, stable cell lines were isolated that expressed ZAP70, Syk, CD45, ZAP70 plus CD45, and Syk plus CD45. IgE stimulation did not induce degranulation in parental double-deficient cells, nor in the cells expressing only CD45. ZAP70 expression did not restore FcεRI signaling unless CD45 was coexpressed in the cells. However, Syk alone restored the IgE signal transduction pathway. The coexpression of CD45 with Syk had no significant effects on the responses to FcεRI-aggregation. There was much better binding of Syk than ZAP70 to the phosphorylated FcεRIγ-ITAM. Furthermore, unlike Syk, ZAP70 required CD45 to display receptor-induced increase in kinase activity. Therefore, in mast cells, ZAP70, but not Syk, requires CD45 for Ag receptor-induced signaling." @default.
- W4313347815 created "2023-01-06" @default.
- W4313347815 creator A5045703368 @default.
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- W4313347815 date "1999-09-01" @default.
- W4313347815 modified "2023-10-13" @default.
- W4313347815 title "CD45 Is Essential for FcεRI Signaling by ZAP70, But Not Syk, in Syk-Negative Mast Cells" @default.
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- W4313347815 doi "https://doi.org/10.4049/jimmunol.163.5.2508" @default.
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