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- W4313349600 abstract "Abstract It is well going to know that several particles modulate immune responses and phagocytosis may induce activation in antigen-presenting cells. Asian sand dust (ASD), which is derived from air pollutants in East China that migrate to Japan and ASD is concerned about influence for health. Our group has reported that ASD induced inflammatory responses in lung tissue and causes respiratory diseases in mice. In the last meeting, we reported that the effect of ASD on the immune system in vivo. In this study, we examined the effect of ASD in vitro. Cell lysates were prepared from freshly isolated splenocytes of ICR mice and resolved by western blotting. Immunoblotting demonstrated that ASD induced autophagic-marker LC3-II expression and decreased the level of p62 especially in CD11b+ cells. Cell activation of splenocytes was evaluated by a cell viability assay. The cell viability assay showed mitogen-induced proliferation of splenocytes is inhibited by ASD treatment. In addition, an electrophoretic mobility shift assay demonstrated increased activated-NF-κB in bone marrow derived macrophage after ASD treatment. Taken together, these results suggest that ASD activates immune cells, and may modulate cell survival or activity." @default.
- W4313349600 created "2023-01-06" @default.
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- W4313349600 date "2013-05-01" @default.
- W4313349600 modified "2023-09-27" @default.
- W4313349600 title "Asian sand dust induces autophagic markers and activates NF-κB in immune cells (P6377)" @default.
- W4313349600 doi "https://doi.org/10.4049/jimmunol.190.supp.201.6" @default.
- W4313349600 hasPublicationYear "2013" @default.
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