Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313349871> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W4313349871 endingPage "154.35" @default.
- W4313349871 startingPage "154.35" @default.
- W4313349871 abstract "Abstract Cytotoxic T lymphocytes (CTL) are the major killer of virus-infected cells. Granzyme B (GrB) from CTL induces apoptosis in target cells by cleavage and activation of substrates such as caspase-3. However, while undergoing apoptosis, cells can still produce infectious viruses unless a mechanism exists to specifically inhibit viral production. Using proteomic approaches, we found a novel GrB target that plays a major role in protein synthesis: eukaryotic initiation factor 4 Gamma 3 (eIF4G3). We hypothesized a novel role for GrB to arrest translation of viral proteins by targeting eIF4G3, which contains the GrB recognition sequence IESD1408S. We showed that GrB specifically cleaves eIF4G3 since the mutant eIF4G-Δ, with Ala substituted for D1408, was resistant to GrB degradation. Translational inhibition by GrB was reversed by the inclusion of eIF4G-Δ, showing that eIF4G3 is the critical GrB substrate involved in translation. In Jurkat cells, GrB or CTL treatment resulted in reduced rate of translation. We infected Jurkat cells with vaccinia virus (VV) and showed that viral protein synthesis and viral replication was inhibited in response to GrB or CTL. Transfection of eIF4G-Δ reversed the halt in protein synthesis induced by GrB. This demonstrated for the first time that GrB could prevent the production of VV by targeting the translational machinery of the host. These results represent a major new insight into what constitutes an effective CTL response to a virus-infected cell." @default.
- W4313349871 created "2023-01-06" @default.
- W4313349871 creator A5016720351 @default.
- W4313349871 creator A5019957951 @default.
- W4313349871 creator A5021866589 @default.
- W4313349871 creator A5026845136 @default.
- W4313349871 creator A5041016688 @default.
- W4313349871 creator A5046174493 @default.
- W4313349871 creator A5068172568 @default.
- W4313349871 creator A5068188462 @default.
- W4313349871 date "2011-04-01" @default.
- W4313349871 modified "2023-09-30" @default.
- W4313349871 title "Granzyme B inhibits translation of viral proteins and viral replication through proteolytic cleavage of the eukaryotic initiation factor 4 gamma 3 (154.35)" @default.
- W4313349871 doi "https://doi.org/10.4049/jimmunol.186.supp.154.35" @default.
- W4313349871 hasPublicationYear "2011" @default.
- W4313349871 type Work @default.
- W4313349871 citedByCount "0" @default.
- W4313349871 crossrefType "journal-article" @default.
- W4313349871 hasAuthorship W4313349871A5016720351 @default.
- W4313349871 hasAuthorship W4313349871A5019957951 @default.
- W4313349871 hasAuthorship W4313349871A5021866589 @default.
- W4313349871 hasAuthorship W4313349871A5026845136 @default.
- W4313349871 hasAuthorship W4313349871A5041016688 @default.
- W4313349871 hasAuthorship W4313349871A5046174493 @default.
- W4313349871 hasAuthorship W4313349871A5068172568 @default.
- W4313349871 hasAuthorship W4313349871A5068188462 @default.
- W4313349871 hasConcept C104317684 @default.
- W4313349871 hasConcept C105580179 @default.
- W4313349871 hasConcept C140704245 @default.
- W4313349871 hasConcept C147969180 @default.
- W4313349871 hasConcept C149364088 @default.
- W4313349871 hasConcept C153911025 @default.
- W4313349871 hasConcept C154317977 @default.
- W4313349871 hasConcept C159047783 @default.
- W4313349871 hasConcept C179464577 @default.
- W4313349871 hasConcept C202751555 @default.
- W4313349871 hasConcept C203014093 @default.
- W4313349871 hasConcept C2522874641 @default.
- W4313349871 hasConcept C2776054693 @default.
- W4313349871 hasConcept C2776090121 @default.
- W4313349871 hasConcept C2780380082 @default.
- W4313349871 hasConcept C55493867 @default.
- W4313349871 hasConcept C86803240 @default.
- W4313349871 hasConcept C8891405 @default.
- W4313349871 hasConcept C95444343 @default.
- W4313349871 hasConceptScore W4313349871C104317684 @default.
- W4313349871 hasConceptScore W4313349871C105580179 @default.
- W4313349871 hasConceptScore W4313349871C140704245 @default.
- W4313349871 hasConceptScore W4313349871C147969180 @default.
- W4313349871 hasConceptScore W4313349871C149364088 @default.
- W4313349871 hasConceptScore W4313349871C153911025 @default.
- W4313349871 hasConceptScore W4313349871C154317977 @default.
- W4313349871 hasConceptScore W4313349871C159047783 @default.
- W4313349871 hasConceptScore W4313349871C179464577 @default.
- W4313349871 hasConceptScore W4313349871C202751555 @default.
- W4313349871 hasConceptScore W4313349871C203014093 @default.
- W4313349871 hasConceptScore W4313349871C2522874641 @default.
- W4313349871 hasConceptScore W4313349871C2776054693 @default.
- W4313349871 hasConceptScore W4313349871C2776090121 @default.
- W4313349871 hasConceptScore W4313349871C2780380082 @default.
- W4313349871 hasConceptScore W4313349871C55493867 @default.
- W4313349871 hasConceptScore W4313349871C86803240 @default.
- W4313349871 hasConceptScore W4313349871C8891405 @default.
- W4313349871 hasConceptScore W4313349871C95444343 @default.
- W4313349871 hasIssue "1_Supplement" @default.
- W4313349871 hasLocation W43133498711 @default.
- W4313349871 hasOpenAccess W4313349871 @default.
- W4313349871 hasPrimaryLocation W43133498711 @default.
- W4313349871 hasRelatedWork W1506455075 @default.
- W4313349871 hasRelatedWork W1551043459 @default.
- W4313349871 hasRelatedWork W1563157353 @default.
- W4313349871 hasRelatedWork W1964955632 @default.
- W4313349871 hasRelatedWork W1979511395 @default.
- W4313349871 hasRelatedWork W2052483994 @default.
- W4313349871 hasRelatedWork W2076874973 @default.
- W4313349871 hasRelatedWork W2111211532 @default.
- W4313349871 hasRelatedWork W2171066181 @default.
- W4313349871 hasRelatedWork W4313349871 @default.
- W4313349871 hasVolume "186" @default.
- W4313349871 isParatext "false" @default.
- W4313349871 isRetracted "false" @default.
- W4313349871 workType "article" @default.