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- W4313350021 abstract "Abstract Using a micropipette adhesion frequency assay, we are able to determine the affinity of polyclonal and monoclonal T cells without needing to isolate the TCR. This assay allows for the direct measuring of adhesion between a T cell and a surrogate APC, which is a human red blood cell that has been coated with peptide:MHC monomers. Utilizing this two dimensional measure of affinity, we determined the effective affinities for the following CD4+ TCR transgenic clones: 2D2, BDC2.5, and SMARTA for their cognate antigens. For the 2D2 cells, we assessed both MOG38-49:IAb and NF-M18-30:IAb monomers since it has been shown that 2D2 cells can cross-react with both of these CNS antigens. Our results demonstrate a wide range of affinities for these various transgenic cells. The hierarchy of the affinities from highest to lowest for the clones (clone:peptide) was; BDC2.5:1040-63, SMARTA:GP-61, 2D2:NFM18-30 and 2D2:MOG38-49 and roughly correlated with their responsiveness to antigen. The affinities ranged from 10-4 µm4 to less than 10-8 µm4, which is below the limits of detection for this technique. These data demonstrate that there is a wide range of affinities for CD4+ T cells that are capable of responding to antigen." @default.
- W4313350021 created "2023-01-06" @default.
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- W4313350021 date "2011-04-01" @default.
- W4313350021 modified "2023-09-26" @default.
- W4313350021 title "Analysis of several CD4+ T cell clones reveals a >10,000 fold range in 2D affinity of antigen recognition (100.45)" @default.
- W4313350021 doi "https://doi.org/10.4049/jimmunol.186.supp.100.45" @default.
- W4313350021 hasPublicationYear "2011" @default.
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