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• W4313352838 startingPage "629" @default.
• W4313352838 abstract "Plants are always affected by various abiotic and biotic stresses such as microbial infection, salinity, drought, high temperature, cold, heavy metal stress, and oxidative stress. Besides disturbing growth and development, both abiotic and biotic stresses certainly affect the metabolites and the genes involved in producing the metabolites of the plants. As stresses affect the homeostasis of plants, they express different metabolites again to attain metabolic homeostasis. The metabolites produced by plants are free radical scavengers and osmoprotectants, and signalling molecules, such as myo-inositol, mannitol, and dimethylsulfonium compounds like betaine, glycine, and fructose, sucrose, and trehalose sugars, and amino acids. The other molecules, such as tocopherols, ascorbic acid, and anthocyanins. Jasmonic acid, salicylic acid, methyl salicylate, and methyl jasmonate are the signalling molecules produced during different plant stresses. Various tools are used for profiling and fingerprinting, namely Fourier transform infrared spectroscopy (FTIR), Fourier transform ion cyclotron resonance mass spectroscopy, nuclear magnetic resonance (NMR), mass spectroscopy (MS), liquid chromatography-mass spectroscopy (LC-MS), gas chromatography-mass spectroscopy (GC-MS), capillary electrophoresis-mass spectroscopy (CE-MS), and high-performance liquid chromatography (HPLC). The advantage of metabolomics is that the changes in metabolites can be detected very early before any other characteristics, such as a change in transcription pattern and expression of proteins during stress response as metabolic expression changes. In the genomic approach to study stress biology of plant metabolites, molecular approaches are applied, which deal with the genes responsible for the production of metabolites. As the genome sequences of crops are available now, we can easily elucidate which genes are expressed to produce the metabolites in response to different stress. This is achieved through the profiling of the complete metabolome and linking it with the genome of plants. In structural genomics approaches, the DNA sequence of the plants is determined by manual methods or by robots. Other techniques involved in genomic approaches are microarray analysis, massive parallel signature sequencing (MPSS), qRT-PCR, and serial analysis of gene expression. This chapter discusses the genomics and metabolomic approaches to study and understand the stress biology of plant metabolites. Further it discusses on how plants respond to stresses by producing metabolites, which metabolites are produced and which genes are involved in metabolite production, and the detection methods for genes." @default.
• W4313352838 created "2023-01-06" @default.
• W4313352838 creator A5023617058 @default.
• W4313352838 creator A5027477284 @default.
• W4313352838 creator A5033516796 @default.
• W4313352838 creator A5056515693 @default.
• W4313352838 date "2022-01-01" @default.
• W4313352838 modified "2023-10-18" @default.
• W4313352838 title "Metabolomics and Genomics for Understanding Stress Biology of Plant Metabolites" @default.
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