Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313355978> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4313355978 endingPage "81.16" @default.
- W4313355978 startingPage "81.16" @default.
- W4313355978 abstract "Abstract Characterization of the immune system in remote locations is challenging, requiring technical expertise and specific analytical equipment. Immune functional assessments require live cells, and the window of viability following blood donation is limited. To test the ability of ACD blood collection tubes to expand this window of viability, cellular function was analyzed over 4 consecutive days, beginning on the date of blood collection. T-cell activation, characterized by CD69 and CD25 expression, was measured following a 24-hour stimulation with soluble anti-CD3 and anti-CD28 antibodies (CD3/CD28) or with staphylococcus enterotoxins A and B (SEA+SEB). Mitogen stimulated cytokine profiles were determined following a 48-hour stimulation with CD3/CD28, phytohemagglutinin and ionomycin (PMA+I), or lipopolysaccharide (LPS). T-cell activation following stimulation with SEA+SEB was not altered by the processing delay; however, following stimulation with CD3/CD28, T-cell activation increased over the 72 hour period. The processing delay did not affect cytokine production following CD3/CD28 stimulation. Following stimulation with LPS and PMA+I, results were varied. While some cytokine responses were unaltered following the processing delay, others were impaired. These findings confirm the general viability of whole blood stored at room temperature (68 to 72°F) for 48 to 72 hours in ACD tubes and suggest many phenotypic and functional assays are feasible on blood samples aged as described. Investigators using this protocol should confirm their specific culture technique and measured output. This protocol may assist studies of subjects in remote locations with no immediate access to laboratory instrumentation." @default.
- W4313355978 created "2023-01-06" @default.
- W4313355978 creator A5000814392 @default.
- W4313355978 creator A5021637559 @default.
- W4313355978 creator A5048604529 @default.
- W4313355978 creator A5052130562 @default.
- W4313355978 creator A5091346776 @default.
- W4313355978 date "2017-05-01" @default.
- W4313355978 modified "2023-10-01" @default.
- W4313355978 title "Functional stability of ACD anticoagulated blood samples stored for 72 hours at room temperature." @default.
- W4313355978 doi "https://doi.org/10.4049/jimmunol.198.supp.81.16" @default.
- W4313355978 hasPublicationYear "2017" @default.
- W4313355978 type Work @default.
- W4313355978 citedByCount "0" @default.
- W4313355978 crossrefType "journal-article" @default.
- W4313355978 hasAuthorship W4313355978A5000814392 @default.
- W4313355978 hasAuthorship W4313355978A5021637559 @default.
- W4313355978 hasAuthorship W4313355978A5048604529 @default.
- W4313355978 hasAuthorship W4313355978A5052130562 @default.
- W4313355978 hasAuthorship W4313355978A5091346776 @default.
- W4313355978 hasConcept C114684123 @default.
- W4313355978 hasConcept C126322002 @default.
- W4313355978 hasConcept C148125776 @default.
- W4313355978 hasConcept C1621761 @default.
- W4313355978 hasConcept C16685009 @default.
- W4313355978 hasConcept C167672396 @default.
- W4313355978 hasConcept C176324957 @default.
- W4313355978 hasConcept C203014093 @default.
- W4313355978 hasConcept C24998067 @default.
- W4313355978 hasConcept C2776090121 @default.
- W4313355978 hasConcept C2776989580 @default.
- W4313355978 hasConcept C2778690821 @default.
- W4313355978 hasConcept C71924100 @default.
- W4313355978 hasConcept C79484868 @default.
- W4313355978 hasConcept C86803240 @default.
- W4313355978 hasConcept C8891405 @default.
- W4313355978 hasConceptScore W4313355978C114684123 @default.
- W4313355978 hasConceptScore W4313355978C126322002 @default.
- W4313355978 hasConceptScore W4313355978C148125776 @default.
- W4313355978 hasConceptScore W4313355978C1621761 @default.
- W4313355978 hasConceptScore W4313355978C16685009 @default.
- W4313355978 hasConceptScore W4313355978C167672396 @default.
- W4313355978 hasConceptScore W4313355978C176324957 @default.
- W4313355978 hasConceptScore W4313355978C203014093 @default.
- W4313355978 hasConceptScore W4313355978C24998067 @default.
- W4313355978 hasConceptScore W4313355978C2776090121 @default.
- W4313355978 hasConceptScore W4313355978C2776989580 @default.
- W4313355978 hasConceptScore W4313355978C2778690821 @default.
- W4313355978 hasConceptScore W4313355978C71924100 @default.
- W4313355978 hasConceptScore W4313355978C79484868 @default.
- W4313355978 hasConceptScore W4313355978C86803240 @default.
- W4313355978 hasConceptScore W4313355978C8891405 @default.
- W4313355978 hasIssue "1_Supplement" @default.
- W4313355978 hasLocation W43133559781 @default.
- W4313355978 hasOpenAccess W4313355978 @default.
- W4313355978 hasPrimaryLocation W43133559781 @default.
- W4313355978 hasRelatedWork W1558126419 @default.
- W4313355978 hasRelatedWork W1977613415 @default.
- W4313355978 hasRelatedWork W2000107960 @default.
- W4313355978 hasRelatedWork W2071733190 @default.
- W4313355978 hasRelatedWork W2072719268 @default.
- W4313355978 hasRelatedWork W2077374904 @default.
- W4313355978 hasRelatedWork W2088171781 @default.
- W4313355978 hasRelatedWork W2418681771 @default.
- W4313355978 hasRelatedWork W4313354819 @default.
- W4313355978 hasRelatedWork W789762825 @default.
- W4313355978 hasVolume "198" @default.
- W4313355978 isParatext "false" @default.
- W4313355978 isRetracted "false" @default.
- W4313355978 workType "article" @default.