Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313356406> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W4313356406 endingPage "186.12" @default.
- W4313356406 startingPage "186.12" @default.
- W4313356406 abstract "Abstract CD4+ T cell responses are thought to play a key role in the progression of type 1 diabetes (T1D). However, effective monitoring and characterization of T cells that respond to beta cell epitopes in subjects with T1D has been limited by technical obstacles, including the inherently low frequencies of autoreactive CD4+ T cells and the variable responsiveness of individual subjects to single epitopes. We have recently implemented a combinatorial staining approach that allows direct ex vivo characterization of multiple CD4+ T-cell specificities in a single sample. We applied this combinatorial HLA class II multimer assay to directly measure the frequency and phenotype of beta cell specific CD4+ T cells. For this work we utilized five peptides that were previously identified as naturally processed DRB1*04:01 restricted epitopes from proinsulin, GAD65, IA-2, and IGRP. Although responses to each of these peptides can be readily detected after in vitro expansion culture, our results indicated that only proinsulin specific T cells were consistently present at high frequencies in subjects with T1D. Phenotypic analysis of beta cell specific CD4+ T cells revealed that subjects with T1D had a higher frequency of proinsulin specific T cells with a memory phenotype than HLA matched controls. The majority of these memory cells were CXCR3 positive and an increased percentage were CCR7 negative, suggesting that Th1-like effector memory responses are present in subjects with T1D. Finally, this approach is compatible with combinatorial class I multimer analysis, facilitating the characterization of self-reactive CD4+ and CD8+ T cells using a single sample." @default.
- W4313356406 created "2023-01-06" @default.
- W4313356406 creator A5019036735 @default.
- W4313356406 creator A5025070212 @default.
- W4313356406 creator A5038501139 @default.
- W4313356406 creator A5073087501 @default.
- W4313356406 creator A5090475940 @default.
- W4313356406 date "2016-05-01" @default.
- W4313356406 modified "2023-10-17" @default.
- W4313356406 title "Combinatorial ex vivo analysis of beta cell specific CD4+ T-cell responses reveals a predominance of proinsulin specific cells" @default.
- W4313356406 doi "https://doi.org/10.4049/jimmunol.196.supp.186.12" @default.
- W4313356406 hasPublicationYear "2016" @default.
- W4313356406 type Work @default.
- W4313356406 citedByCount "0" @default.
- W4313356406 crossrefType "journal-article" @default.
- W4313356406 hasAuthorship W4313356406A5019036735 @default.
- W4313356406 hasAuthorship W4313356406A5025070212 @default.
- W4313356406 hasAuthorship W4313356406A5038501139 @default.
- W4313356406 hasAuthorship W4313356406A5073087501 @default.
- W4313356406 hasAuthorship W4313356406A5090475940 @default.
- W4313356406 hasConcept C134018914 @default.
- W4313356406 hasConcept C147483822 @default.
- W4313356406 hasConcept C148125776 @default.
- W4313356406 hasConcept C153911025 @default.
- W4313356406 hasConcept C154317977 @default.
- W4313356406 hasConcept C167672396 @default.
- W4313356406 hasConcept C195616568 @default.
- W4313356406 hasConcept C199360897 @default.
- W4313356406 hasConcept C202751555 @default.
- W4313356406 hasConcept C203014093 @default.
- W4313356406 hasConcept C26291073 @default.
- W4313356406 hasConcept C2776090121 @default.
- W4313356406 hasConcept C2776174256 @default.
- W4313356406 hasConcept C2777367678 @default.
- W4313356406 hasConcept C2779306644 @default.
- W4313356406 hasConcept C41008148 @default.
- W4313356406 hasConcept C55493867 @default.
- W4313356406 hasConcept C86803240 @default.
- W4313356406 hasConcept C8891405 @default.
- W4313356406 hasConcept C90061646 @default.
- W4313356406 hasConcept C95444343 @default.
- W4313356406 hasConceptScore W4313356406C134018914 @default.
- W4313356406 hasConceptScore W4313356406C147483822 @default.
- W4313356406 hasConceptScore W4313356406C148125776 @default.
- W4313356406 hasConceptScore W4313356406C153911025 @default.
- W4313356406 hasConceptScore W4313356406C154317977 @default.
- W4313356406 hasConceptScore W4313356406C167672396 @default.
- W4313356406 hasConceptScore W4313356406C195616568 @default.
- W4313356406 hasConceptScore W4313356406C199360897 @default.
- W4313356406 hasConceptScore W4313356406C202751555 @default.
- W4313356406 hasConceptScore W4313356406C203014093 @default.
- W4313356406 hasConceptScore W4313356406C26291073 @default.
- W4313356406 hasConceptScore W4313356406C2776090121 @default.
- W4313356406 hasConceptScore W4313356406C2776174256 @default.
- W4313356406 hasConceptScore W4313356406C2777367678 @default.
- W4313356406 hasConceptScore W4313356406C2779306644 @default.
- W4313356406 hasConceptScore W4313356406C41008148 @default.
- W4313356406 hasConceptScore W4313356406C55493867 @default.
- W4313356406 hasConceptScore W4313356406C86803240 @default.
- W4313356406 hasConceptScore W4313356406C8891405 @default.
- W4313356406 hasConceptScore W4313356406C90061646 @default.
- W4313356406 hasConceptScore W4313356406C95444343 @default.
- W4313356406 hasIssue "1_Supplement" @default.
- W4313356406 hasLocation W43133564061 @default.
- W4313356406 hasOpenAccess W4313356406 @default.
- W4313356406 hasPrimaryLocation W43133564061 @default.
- W4313356406 hasRelatedWork W1528787689 @default.
- W4313356406 hasRelatedWork W2001342405 @default.
- W4313356406 hasRelatedWork W2012986510 @default.
- W4313356406 hasRelatedWork W2066889665 @default.
- W4313356406 hasRelatedWork W2102107932 @default.
- W4313356406 hasRelatedWork W2140041178 @default.
- W4313356406 hasRelatedWork W2164750677 @default.
- W4313356406 hasRelatedWork W2370976773 @default.
- W4313356406 hasRelatedWork W2801946978 @default.
- W4313356406 hasRelatedWork W2783849630 @default.
- W4313356406 hasVolume "196" @default.
- W4313356406 isParatext "false" @default.
- W4313356406 isRetracted "false" @default.
- W4313356406 workType "article" @default.