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- W4313356460 startingPage "53.4" @default.
- W4313356460 abstract "Abstract Exposure to aeroallergens is a major risk factor for asthma development. Allergen possessing proteolytic activity is capable of disrupting airway epithelium. Increasing evidence pointing to asthma as an epithelial disease, however, the underlying mechanisms that drive asthma are not fully elucidated. Here, we investigated the DNA damage potential of aeroallergens on human bronchial epithelial cells BEAS-2B, and elucidated the damaging mechanisms. We exposed BEAS-2B cells to house dust mite (HDM), and measured DNA damage by CometChip and DNA double strand breaks (DSBs) by γH2AX staining. Intracellular oxidative stress was evaluated by staining cells with CellROS and MitoTracker. Antioxidant genes expression were determined using RT-PCR. Cell viability was determined by XTT assay. Cell death was studied using flow cytometry. To elucidate the underlying mechanism, cells were treated with Toll-like receptor 4 (TLR4) agonist or antagonist, or antioxidants glutathione or catalase. BEAS-2B cells exposed to HDM showed enhanced DNA damage, elevated ROS production, and increased mitochondrial oxidative stress and nitrite level. Expression of Nrf2-dependent antioxidant genes was reduced by HDM exposure. HDM also reduced cell viability and induced cell death. Importantly, HDM-induced DNA damage could be prevented by antioxidants. Our results suggest that direct HDM exposure to bronchial epithelial cells leads to production of RONS that damage DNA and induce cytotoxicity. Antioxidants can prevent HDM-induced DNA damage, revealing a novel role for antioxidants in preventing genotoxicity. [This study was partially supported by NMRC/CBRG/0027/2012, NUHS Seed Grant R-184-000-238-112, NRF, NIEHS R44-ES0211116 and Trevigen Inc.]" @default.
- W4313356460 created "2023-01-06" @default.
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- W4313356460 date "2016-05-01" @default.
- W4313356460 modified "2023-10-14" @default.
- W4313356460 title "Direct aeroallergen exposure disrupts the oxidant and antioxidant balance in human bronchial epithelial cells" @default.
- W4313356460 doi "https://doi.org/10.4049/jimmunol.196.supp.53.4" @default.
- W4313356460 hasPublicationYear "2016" @default.
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