Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313358910> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W4313358910 endingPage "131.9" @default.
- W4313358910 startingPage "131.9" @default.
- W4313358910 abstract "Abstract Immunomagnetic cell separation is a powerful tool of immunology, however the presence of beads on the cell surface might alter the electrophysiological and pharmacological parameters of the ion channels expressed in the separated cells. We addressed this issue by measuring the aforementioned properties of Kv1.3, the predominant voltage-gated K+ channel of activated T cells upon immunomagnetic separation. PBMCs from healthy donors were activated then CD4+ cells were separated with REAlease® CD4 Kit (Miltenyi Biotec Inc.). As control, we made stepwise removal of REAlease® antibody-bead complex, immunomagnetic negative separation and FACS. Purity and viability was determined by flow cytometry. Electrophysiology studies were made with patch-clamp technique in whole-cell configuration. The presence of bead-antibody complex statistically significantly increased the time constant (τact) characterizing the activation kinetics of the current as compared to negative selection (p=0.049) and antibody only (p=0.024) controls, however the increase in τact from ~0.5 ms to ~0.6 ms does not bear any biological significance. Other gating parameters of Kv1.3, such as inactivation time constant and midpoint of voltage-dependence of steady-state activation were unaffected by the beads. Moreover, the binding of a peptide (ChTx) and a small organic molecule (TEA) inhibitor to Kv1.3 was oblivious to the presence of beads. In addition, the electrical capacitance of the cell membrane was not altered when beads were attached to the cells. Based on our results we conclude that the application of immunomagnetic cell separation is completely suitable to electrophysiology studies of immune cells." @default.
- W4313358910 created "2023-01-06" @default.
- W4313358910 creator A5016412232 @default.
- W4313358910 creator A5018058852 @default.
- W4313358910 creator A5036677862 @default.
- W4313358910 creator A5054497219 @default.
- W4313358910 creator A5065118109 @default.
- W4313358910 creator A5091098124 @default.
- W4313358910 date "2019-05-01" @default.
- W4313358910 modified "2023-09-28" @default.
- W4313358910 title "Immunomagnetic cell separation is a suitable method for T cell electrophysiology and ion channel pharmacology" @default.
- W4313358910 doi "https://doi.org/10.4049/jimmunol.202.supp.131.9" @default.
- W4313358910 hasPublicationYear "2019" @default.
- W4313358910 type Work @default.
- W4313358910 citedByCount "0" @default.
- W4313358910 crossrefType "journal-article" @default.
- W4313358910 hasAuthorship W4313358910A5016412232 @default.
- W4313358910 hasAuthorship W4313358910A5018058852 @default.
- W4313358910 hasAuthorship W4313358910A5036677862 @default.
- W4313358910 hasAuthorship W4313358910A5054497219 @default.
- W4313358910 hasAuthorship W4313358910A5065118109 @default.
- W4313358910 hasAuthorship W4313358910A5091098124 @default.
- W4313358910 hasConcept C12554922 @default.
- W4313358910 hasConcept C153911025 @default.
- W4313358910 hasConcept C169760540 @default.
- W4313358910 hasConcept C170493617 @default.
- W4313358910 hasConcept C181911157 @default.
- W4313358910 hasConcept C185263204 @default.
- W4313358910 hasConcept C185592680 @default.
- W4313358910 hasConcept C194544171 @default.
- W4313358910 hasConcept C2781053203 @default.
- W4313358910 hasConcept C43617362 @default.
- W4313358910 hasConcept C50254741 @default.
- W4313358910 hasConcept C553184892 @default.
- W4313358910 hasConcept C55493867 @default.
- W4313358910 hasConcept C86803240 @default.
- W4313358910 hasConceptScore W4313358910C12554922 @default.
- W4313358910 hasConceptScore W4313358910C153911025 @default.
- W4313358910 hasConceptScore W4313358910C169760540 @default.
- W4313358910 hasConceptScore W4313358910C170493617 @default.
- W4313358910 hasConceptScore W4313358910C181911157 @default.
- W4313358910 hasConceptScore W4313358910C185263204 @default.
- W4313358910 hasConceptScore W4313358910C185592680 @default.
- W4313358910 hasConceptScore W4313358910C194544171 @default.
- W4313358910 hasConceptScore W4313358910C2781053203 @default.
- W4313358910 hasConceptScore W4313358910C43617362 @default.
- W4313358910 hasConceptScore W4313358910C50254741 @default.
- W4313358910 hasConceptScore W4313358910C553184892 @default.
- W4313358910 hasConceptScore W4313358910C55493867 @default.
- W4313358910 hasConceptScore W4313358910C86803240 @default.
- W4313358910 hasIssue "1_Supplement" @default.
- W4313358910 hasLocation W43133589101 @default.
- W4313358910 hasOpenAccess W4313358910 @default.
- W4313358910 hasPrimaryLocation W43133589101 @default.
- W4313358910 hasRelatedWork W1622221132 @default.
- W4313358910 hasRelatedWork W2022753918 @default.
- W4313358910 hasRelatedWork W2089774470 @default.
- W4313358910 hasRelatedWork W2291957848 @default.
- W4313358910 hasRelatedWork W2307938811 @default.
- W4313358910 hasRelatedWork W2384512550 @default.
- W4313358910 hasRelatedWork W2909181336 @default.
- W4313358910 hasRelatedWork W3000026933 @default.
- W4313358910 hasRelatedWork W3036129729 @default.
- W4313358910 hasRelatedWork W3130225787 @default.
- W4313358910 hasVolume "202" @default.
- W4313358910 isParatext "false" @default.
- W4313358910 isRetracted "false" @default.
- W4313358910 workType "article" @default.