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• W4313361502 endingPage "72" @default.
• W4313361502 startingPage "72" @default.
• W4313361502 abstract "Microbial transformation of hydrophobic phytosterols into the pharmaceutical steroid precursors AD (androst-4-ene-3, 17-dione) and ADD (androst-4-diene-3, 17-dione) in a water–plant oil two-phase system by Mycolicibacterium neoaurum is a paradigm of interfacial biocatalysis in Pickering emulsions stabilized by bacterial cells. In the present work, phase inversion of Pickering emulsions—i.e., Pickering emulsions turning from water-in-oil (W/O) emulsions into oil-in-water (O/W) ones—was observed during microbial transformation in the presence of high concentrations of crystal phytosterols. It was found that there is a correlation relationship between the phase behaviors of Pickering emulsions and the biocatalytic activity of utilizing M. neoaurum as a whole-cell catalyst. Efficient microbial transformation under the high crystal phytosterol loadings was achieved due to the formation of O/W emulsions where interfacial biocatalysis took place. Under the optimal conditions (volume ratio of soybean oil to water: 15:35 mL, phytosterols concentration in the soybean oil: 80 g/L, glucose as co-substrate in the aqueous culture medium: 10 g/L), the concentrations of AD and ADD reached 4.8 g/L based on the whole broth (16 g/L based on the oil phase) after microbial transformation for 9 days." @default.
• W4313361502 created "2023-01-06" @default.
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• W4313361502 date "2022-12-30" @default.
• W4313361502 modified "2023-09-25" @default.
• W4313361502 title "Correlation Relationship between Phase Inversion of Pickering Emulsions and Biocatalytic Activity of Microbial Transformation of Phytosterols" @default.
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• W4313361502 doi "https://doi.org/10.3390/catal13010072" @default.
• W4313361502 hasPublicationYear "2022" @default.
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