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- W4313366013 abstract "Abstract The ocular surface is a mucosal site that interacts with the environment and protects the eye. We previously identified an ocular surface commensal, Corynebacterium mastitidis (C. mast.), which induced IL-17A production from γδ T cells in the conjunctiva, affording protection from infection. In the current study we dissect the requirement for IL-17A production in response to C. mast by Vγ4+and Vγ4−γδ T cell subsets. We found that colonization of C. mast-negative mice with C. mast. increased the frequency of IL-17A+ cells in eye-draining lymph nodes by several fold in both subsets. However, whereas Vγ4−γδ T cells upregulated their expression of IL-1R, Vγ4+γδ cells downregulated their CD3 expression, possibly secondary to TCR engagement. To examine this further, we performed in vitro co-cultures of dendritic cells and γδ T cells. With C. mast as the sole stimulant, only Vγ4+ cells, but not Vγ4− cells, produced IL-17A. Addition of exogenous IL1 to the co-cultures elicited production of IL-17A also from the Vγ4− subset, whereas inhibition of downstream TCR signaling events by FK-506 or CsA abrogated IL-17A production in the Vγ4+ subset. Furthermore, C. mast produced a positive Nur77-GFP signal only in the Vγ4+ subset. IL-1R blocking antibody abrogated IL-17A production in both subsets. We propose that Vγ4+and Vγ4− subsets may sense and respond to C. mast by IL-17A production using distinct pathways. Although an IL-1R signal is needed by both subsets, their level of dependence on this signal differs. IL-1R signaling is dominant in Vγ4−γδ T cells, whose TCR does not appear to respond to C. mast. In contrast, a much lower level of IL-1 appears to be sufficient for triggering IL-17A production from Vγ4+ γδ T cells, which recognize C. mast via their TCR." @default.
- W4313366013 created "2023-01-06" @default.
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- W4313366013 date "2019-05-01" @default.
- W4313366013 modified "2023-09-26" @default.
- W4313366013 title "Production of IL-17A by different subsets of γδ T cells in response to the commensal Corynebacterium mastitidis has distinct requirements for IL-1R and TCR recognition" @default.
- W4313366013 doi "https://doi.org/10.4049/jimmunol.202.supp.190.54" @default.
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