Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313366128> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4313366128 endingPage "64.10" @default.
- W4313366128 startingPage "64.10" @default.
- W4313366128 abstract "Abstract Pattern recognition receptors (PRRs) are nearly ubiquitous in immune and non-immune mammalian cells. PRRs detect molecular pattern molecules associated with pathogen invasion as well as internal damage markers. A subset of pattern-recognition receptors, found endosomally or cytosolically, detect nucleic acids including DNA. Recently, a number of cytosolic DNA-specific PRRs expressed in non-immune cells have been described. Binding and activation of these sensors induces the production of Type I interferons and pro-inflammatory cytokines and chemokines. Many cell types, including B16F10 mouse melanoma cells, respond to DNA electroporation with the upregulation of specific PRRs on the mRNA and protein levels. We developed B16F10 melanoma cell hydrogels to test whether cells in these constructs (“3D cells”) responded similarly to cells in suspension. The cells formed small spheroids within the hydrogels, similar to in vivo tumor morphology, cell-cell, and cell-matrix microenvironment. 3D cells maintained a nearly 73% proliferation rate compared to a 94% rate for cells cultured in 2D monolayers. The relative metabolism kinetics of 3D cells were similar to monolayers in culture, as was transgene expression after delivery by electroporation. However, while similar PRR mRNAs were upregulated, including DAI/ZBP1, DDX60, and p204, the relative levels of upregulation differed significantly between suspended B16F10 and 3D cells. In addition, p204 was upregulated in control constructs but not in suspension cells receiving pulses without DNA. Supporting these observations, the induced cytokine profile also differed. These data emphasize the importance of testing multiple models before generalizing to tissue responses." @default.
- W4313366128 created "2023-01-06" @default.
- W4313366128 creator A5004350639 @default.
- W4313366128 creator A5021065744 @default.
- W4313366128 creator A5048547302 @default.
- W4313366128 creator A5068436889 @default.
- W4313366128 creator A5070257255 @default.
- W4313366128 date "2019-05-01" @default.
- W4313366128 modified "2023-09-26" @default.
- W4313366128 title "Nucleic acid sensing in a 3D hydrogel model of B16F10 mouse melanoma" @default.
- W4313366128 doi "https://doi.org/10.4049/jimmunol.202.supp.64.10" @default.
- W4313366128 hasPublicationYear "2019" @default.
- W4313366128 type Work @default.
- W4313366128 citedByCount "0" @default.
- W4313366128 crossrefType "journal-article" @default.
- W4313366128 hasAuthorship W4313366128A5004350639 @default.
- W4313366128 hasAuthorship W4313366128A5021065744 @default.
- W4313366128 hasAuthorship W4313366128A5048547302 @default.
- W4313366128 hasAuthorship W4313366128A5068436889 @default.
- W4313366128 hasAuthorship W4313366128A5070257255 @default.
- W4313366128 hasConcept C104317684 @default.
- W4313366128 hasConcept C111289621 @default.
- W4313366128 hasConcept C127561419 @default.
- W4313366128 hasConcept C13373296 @default.
- W4313366128 hasConcept C136449434 @default.
- W4313366128 hasConcept C153911025 @default.
- W4313366128 hasConcept C170493617 @default.
- W4313366128 hasConcept C186852380 @default.
- W4313366128 hasConcept C203014093 @default.
- W4313366128 hasConcept C2777658100 @default.
- W4313366128 hasConcept C502942594 @default.
- W4313366128 hasConcept C54009773 @default.
- W4313366128 hasConcept C54355233 @default.
- W4313366128 hasConcept C55493867 @default.
- W4313366128 hasConcept C81885089 @default.
- W4313366128 hasConcept C86803240 @default.
- W4313366128 hasConcept C8891405 @default.
- W4313366128 hasConcept C95444343 @default.
- W4313366128 hasConceptScore W4313366128C104317684 @default.
- W4313366128 hasConceptScore W4313366128C111289621 @default.
- W4313366128 hasConceptScore W4313366128C127561419 @default.
- W4313366128 hasConceptScore W4313366128C13373296 @default.
- W4313366128 hasConceptScore W4313366128C136449434 @default.
- W4313366128 hasConceptScore W4313366128C153911025 @default.
- W4313366128 hasConceptScore W4313366128C170493617 @default.
- W4313366128 hasConceptScore W4313366128C186852380 @default.
- W4313366128 hasConceptScore W4313366128C203014093 @default.
- W4313366128 hasConceptScore W4313366128C2777658100 @default.
- W4313366128 hasConceptScore W4313366128C502942594 @default.
- W4313366128 hasConceptScore W4313366128C54009773 @default.
- W4313366128 hasConceptScore W4313366128C54355233 @default.
- W4313366128 hasConceptScore W4313366128C55493867 @default.
- W4313366128 hasConceptScore W4313366128C81885089 @default.
- W4313366128 hasConceptScore W4313366128C86803240 @default.
- W4313366128 hasConceptScore W4313366128C8891405 @default.
- W4313366128 hasConceptScore W4313366128C95444343 @default.
- W4313366128 hasIssue "1_Supplement" @default.
- W4313366128 hasLocation W43133661281 @default.
- W4313366128 hasOpenAccess W4313366128 @default.
- W4313366128 hasPrimaryLocation W43133661281 @default.
- W4313366128 hasRelatedWork W199846060 @default.
- W4313366128 hasRelatedWork W2021032653 @default.
- W4313366128 hasRelatedWork W2141284323 @default.
- W4313366128 hasRelatedWork W2371788052 @default.
- W4313366128 hasRelatedWork W2381571614 @default.
- W4313366128 hasRelatedWork W2385919261 @default.
- W4313366128 hasRelatedWork W2388976704 @default.
- W4313366128 hasRelatedWork W2392518530 @default.
- W4313366128 hasRelatedWork W2898568897 @default.
- W4313366128 hasRelatedWork W3031992762 @default.
- W4313366128 hasVolume "202" @default.
- W4313366128 isParatext "false" @default.
- W4313366128 isRetracted "false" @default.
- W4313366128 workType "article" @default.