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- W4313366141 abstract "Abstract Flow cytometers are standard tools of the immunologist, providing simultaneous measurement of many targets. The performance of a flow cytometer may vary between instruments, or over time within the same instrument. Evaluation of cytometer performance is essential to ensure results are consistent over time within the same instrument for longitudinal studies and day-to-day data consistency, and across multiple instruments when more than one cytometer is being utilized. Basic capabilities of each fluorescent detector can be estimated using the Q & B calculations, where Q is detection efficiency and B is background. Precision of measurements and minimum detectable dye can be derived from Q & B measurements, to evaluate sensitivity and stability. In this study hard-dyed multi-level, multi-dye beads were collected in three different identically configured cytometers over 11 days. Each day data was collected in 14 different fluorescent detectors at six flow rates. An automated approach to data analysis was utilized to provide unbiased evaluation. All data files were first evaluated with flowCut to check for spurious events. Using automated gating, beads were then identified using Forward Scatter vs. Side Scatter, and flowQB was used to calculate Q & B. This data was used to evaluate intra-instrument and inter-instrument sensitivity and stability. Results show the minimum amount of detectable dye and precision of its measurement are robust across time and different cytometers, though decreased at the highest flow rates of ≥ 500 μL/min. Using an automated unbiased approach for determining Q & B demonstrates cytometer sensitivity and consistency over time, between instruments, and across flow rates." @default.
- W4313366141 created "2023-01-06" @default.
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- W4313366141 date "2019-05-01" @default.
- W4313366141 modified "2023-09-26" @default.
- W4313366141 title "Evaluation of Cytometer Sensitivity and Stability using Automated Analysis" @default.
- W4313366141 doi "https://doi.org/10.4049/jimmunol.202.supp.131.41" @default.
- W4313366141 hasPublicationYear "2019" @default.
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