FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313367459> ?p ?o ?g. }

• W4313367459 endingPage "583" @default.
• W4313367459 startingPage "581" @default.
• W4313367459 abstract "The current protocol for malaria screening in the majority of New South Wales Health Pathology (NSWHP) laboratories is to test the samples for malaria specific antigen using rapid diagnostic tests (RDT). These laboratories refer the RDT negative samples alongside the blood films for confirmatory testing at the malaria reference laboratory, Parasitology Unit, NSW Health Pathology - Institute of Clinical Pathology and Medical Research (ICPMR). Likewise, all blood samples and films with positive RDT are also referred to the malaria reference laboratory for confirmation and identification of the malaria species. Although easy to perform, RDT tests suffer from lack of analytical sensitivity especially for non-falciparum malaria.1Murray C. Gasser R. Magill A. Miller R. Update on rapid diagnostic testing for malaria.Clinl Microbiol Rev. 2008; 21: 97-110Crossref PubMed Scopus (354) Google Scholar Furthermore, deletion of the histadine rich protein (HRP) gene by Plasmodium falciparum is an increasing problem when malaria diagnosis relies on detecting this antigen.2Prosser C. Gresty K. Ellis J. et al.Plasmodium falciparum Histidine-Rich Protein 2 and 3 gene deletions in strains from Nigeria, Sudan, and South Sudan.Emerg Infect Dis. 2021; 27: 471-479Crossref PubMed Google Scholar While it is considered the reference gold standard for malaria detection, malaria diagnosis using blood-film microscopy has significant drawbacks.3Vallejo A. Martínez N. González I. Arévalo-Herrera M. Herrera S. Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia.Plos Negl Trop Dis. 2015; 9: e3453Crossref PubMed Scopus (49) Google Scholar,4Malaria diagnosis: memorandum from a WHO meeting.Bull World Health Organ. 1988; 66: 575-594PubMed Google Scholar This method of diagnosis is difficult to incorporate into laboratory automation systems. It is a labour intensive method and it is becoming more difficult to maintain the quality assurance, skills and numbers of trained microscopists as the number of malaria cases declines globally.4Malaria diagnosis: memorandum from a WHO meeting.Bull World Health Organ. 1988; 66: 575-594PubMed Google Scholar This is especially applicable in the context of non-endemic regions like NSW, Australia, which encounters very few positive cases of malaria (January 2018 to July 2022, 181 malaria notifications in NSW).5NSW HealthInfectious diseases data 2022.https://www1.health.nsw.gov.au/IDD/#/MAL/periodGoogle Scholar The declining skill in malaria microscopy increases the chance of missed cases of malaria especially when parasitaemia is low.3Vallejo A. Martínez N. González I. Arévalo-Herrera M. Herrera S. Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia.Plos Negl Trop Dis. 2015; 9: e3453Crossref PubMed Scopus (49) Google Scholar Several molecular tests based on polymerase chain reaction (PCR) assays have been developed to amplify and detect malarial DNA. These tests have demonstrated higher sensitivity than microscopy,6Kimura M. Kaneko O. Liu Q. et al.Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene.Parasitol Int. 1997; 46: 91-95Crossref Scopus (134) Google Scholar however a time consuming DNA extraction may be required prior to PCR amplification. The turnaround time and skill requirements are significant hurdles for putting these assays to routine laboratory use. Also, a malaria PCR assay would need an elaborate laboratory set up with relatively expensive equipment.7Hänscheid T. Grobusch M. How useful is PCR in the diagnosis of malaria?.Trends Parasitol. 2002; 18: 395-398Abstract Full Text Full Text PDF PubMed Scopus (146) Google Scholar The loop-mediated isothermal amplification (LAMP) assay is a comparatively new molecular technique based on isothermal amplification of DNA. The sensitivity of LAMP assays has been demonstrated as comparable to PCR assays for various parasitic DNA detection including assays for malaria, filaria and strongyloidiasis.8Aonuma H. Yoshimura A. Perera N. et al.Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: dirofilaria immitis as a study model.Parasit Vectors. 2009; 2: 15Crossref PubMed Scopus (51) Google Scholar, 9Watts M. Kim R. Ahuja V. et al.Comparison of loop-mediated isothermal amplification and real-time PCR assays for detection of Strongyloides larvae in different specimen matrices.J Clin Microbiol. 2019; 57e01173-18Crossref Scopus (15) Google Scholar, 10Yongkiettrakul S. Jaroenram W. Arunrut N. et al.Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax.Parasitol Int. 2014; 63: 777-784Crossref PubMed Scopus (46) Google Scholar In addition, LAMP assays can have a higher tolerance to inhibition when compared to PCR.10Yongkiettrakul S. Jaroenram W. Arunrut N. et al.Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax.Parasitol Int. 2014; 63: 777-784Crossref PubMed Scopus (46) Google Scholar A malaria LAMP commercial assay (Alethia Malaria LAMP assay; Meridian Bioscience, USA) has become available in Australia. This assay is supplied in a kit format with pre-dispensed reagents to detect malarial DNA at a genus level. It has the advantage of a simple extraction process prior to isothermal incubation with the whole reaction completed within an hour. In addition, this assay has stable reagents without the need for refrigeration. However, the test cannot distinguish between different Plasmodium species. A verification study was conducted at the malaria reference laboratory, Parasitology Unit, NSWHP-ICPMR, Westmead Hospital, NSW, Australia. The objective of this study was to evaluate the performance of the Alethia malaria LAMP assay (referred as the LAMP assay hereinafter) in detecting malaria compared to blood-film microscopy and the BinaxNOW Malaria RDT (Abbott Laboratories, USA) in returned travellers. The other objective of the study was to consider the application of the LAMP assay in the malaria reference laboratory and the core microbiology section at ICPMR for malaria diagnostic testing. The study had ethical approval from the Western Sydney Local Health District - Human Research Ethics Committee (Approval No. AU RED LNR/18/WMEAD/139). Blood samples (n=150) comprising 58 malaria microscopy positive and 92 malaria microscopy negative specimens were tested in this study (Table 1). The malaria microscopy positive samples comprised 28 P. falciparum, 18 P. vivax, seven P. ovale, two P. malariae and one P. knowlesi microscopy positive, with single species infection. An additional two positive malaria samples had mixed infections; one with P. falciparum and P. vivax, while the other had P. falciparum and P. malariae. Malaria microscopy was performed according to standard laboratory protocols,11World Health OrganizationBasic Malaria Microscopy: Part 1. Learner’s Guide.2nd ed. WHO, Geneva2010http://apps.who.int/iris/bitstream/handle/10665/44208/9789241547826_eng.pdf;jsessionid=E818ADD31EF919BA657B5A3839096717?sequence=1Google Scholar and the RDT and the LAMP assay were performed according to the manufacturer's instructions.Table 1Comparison of the RDT and the LAMP assays in reference to malaria microscopyBinaxNow malaria antigen (n=150)Commercial malaria LAMP (n=150)PositiveNegativePositiveNegativeMalaria microscopy (n=150)Positive (n=58)4711580Negative (n=92)1280785Sensitivity % (95% CI)81.0 (68.6–90.1)100.0 (93.8–100.0)Specificity % (95% CI)87.0 (78.3–93.1)92.4 (85.0–96.9)Positive predictive value % (95% CI)79.7 (69.5–87.1)89.2 (80.3–94.4)Negative predictive value % (95% CI)87.9 (80.9–92.6)100.0CI, confidence interval; LAMP, loop-mediated isothermal amplification; RDT, rapid diagnostic test. Open table in a new tab CI, confidence interval; LAMP, loop-mediated isothermal amplification; RDT, rapid diagnostic test. All 58 samples where malaria parasite was detected by microscopy, tested positive by the LAMP assay. Malaria antigen was detected only on 47 of 58 microscopy positive samples using the RDT and 11 samples were classified as false negative for malaria antigen. Out of the 92 microscopy negative samples, seven samples tested positive for malarial DNA by the LAMP assay. Of note, all seven samples were from patients who had malaria microscopy positive results from the recent past, and this finding was indicative of residual malarial nucleic acids. Apart from these seven discrepant results, no false positive results were detected with the LAMP assay. Malaria antigen was detected in 12 of 92 malaria microscopy negative samples using the RDT. While eight of these 12 samples were follow-up samples from previous malaria microscopy positive cases, the other four samples suggested false positive RDT results. The respective positive predictive values for the RDT and the LAMP assay were 79.7% and 89.2%, while the negative predictive values for these assays were 87.9% and 100%. The time required to process each sample from handling to transfer to the LAMP reader for incubation was less than 6–8 minutes. Results were obtained from the instrument reader 40 minutes after starting the run. This turnaround time was longer compared to the RDT (15–20 minutes), but significantly less than microscopy. In our experience, this is an efficient and fast molecular assay to detect malaria. The LAMP assay was able to detect the five major human Plasmodium species with greater sensitivity and specificity than the RDT. This was in agreement with other similar studies published.12De Koninck A. Cnops L. Hofmans M. Jacobs J. Van den Bossche D. Philippé J. Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene® malaria assay in a non-endemic region.Malar J. 2017; 16: 418Crossref PubMed Scopus (31) Google Scholar The LAMP assay will be useful when experienced microscopists are not available. In a malaria non-endemic region like Australia, this test suits the current laboratory workflow as a rapid molecular test with a relatively rapid screening time for malaria diagnosis and exclusion. While an early diagnosis of malaria can be achieved using the LAMP assay, it cannot differentiate between malaria species. Thus, further testing (malaria microscopy or malaria PCR) would be required for species identification. Similarly, this assay cannot identify mixed malaria infections where more than one Plasmodium species may be present in the sample. This information is usually desired for the treatment and management of malaria. The prolonged circulation of Plasmodium spp. DNA renders the malaria LAMP assay unsuitable as a test for clinical cure against malaria. The LAMP assay is being adopted by the Parasitology Unit, NSWHP-ICPMR, to replace the microscopy confirmation of negative RDT test samples and a corresponding testing algorithm has been developed (Fig. 1). With the implementation of the LAMP assay into the laboratory workflow, there will be increased speed and accuracy of malaria diagnosis in the laboratory." @default.
• W4313367459 created "2023-01-06" @default.
• W4313367459 creator A5028439541 @default.
• W4313367459 creator A5054255525 @default.
• W4313367459 creator A5059673724 @default.
• W4313367459 date "2023-06-01" @default.
• W4313367459 modified "2023-09-27" @default.
• W4313367459 title "Verification and implementation of a commercial loop-mediated isothermal amplification (LAMP) assay for malaria testing in a public laboratory in New South Wales, Australia" @default.
• W4313367459 cites W1965344652 @default.
• W4313367459 cites W2029041215 @default.
• W4313367459 cites W2031222215 @default.
• W4313367459 cites W2053287443 @default.
• W4313367459 cites W2089442633 @default.
• W4313367459 cites W2143917555 @default.
• W4313367459 cites W2765268465 @default.
• W4313367459 cites W2911436967 @default.
• W4313367459 cites W3119897346 @default.
• W4313367459 doi "https://doi.org/10.1016/j.pathol.2022.10.010" @default.
• W4313367459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36707320" @default.
• W4313367459 hasPublicationYear "2023" @default.
• W4313367459 type Work @default.
• W4313367459 citedByCount "0" @default.
• W4313367459 crossrefType "journal-article" @default.
• W4313367459 hasAuthorship W4313367459A5028439541 @default.
• W4313367459 hasAuthorship W4313367459A5054255525 @default.
• W4313367459 hasAuthorship W4313367459A5059673724 @default.
• W4313367459 hasBestOaLocation W43133674591 @default.
• W4313367459 hasConcept C114614502 @default.
• W4313367459 hasConcept C121332964 @default.
• W4313367459 hasConcept C133347239 @default.
• W4313367459 hasConcept C184670325 @default.
• W4313367459 hasConcept C203014093 @default.
• W4313367459 hasConcept C2778048844 @default.
• W4313367459 hasConcept C33923547 @default.
• W4313367459 hasConcept C54355233 @default.
• W4313367459 hasConcept C552990157 @default.
• W4313367459 hasConcept C60635243 @default.
• W4313367459 hasConcept C86803240 @default.
• W4313367459 hasConcept C97355855 @default.
• W4313367459 hasConceptScore W4313367459C114614502 @default.
• W4313367459 hasConceptScore W4313367459C121332964 @default.
• W4313367459 hasConceptScore W4313367459C133347239 @default.
• W4313367459 hasConceptScore W4313367459C184670325 @default.
• W4313367459 hasConceptScore W4313367459C203014093 @default.
• W4313367459 hasConceptScore W4313367459C2778048844 @default.
• W4313367459 hasConceptScore W4313367459C33923547 @default.
• W4313367459 hasConceptScore W4313367459C54355233 @default.
• W4313367459 hasConceptScore W4313367459C552990157 @default.
• W4313367459 hasConceptScore W4313367459C60635243 @default.
• W4313367459 hasConceptScore W4313367459C86803240 @default.
• W4313367459 hasConceptScore W4313367459C97355855 @default.
• W4313367459 hasIssue "4" @default.
• W4313367459 hasLocation W43133674591 @default.
• W4313367459 hasLocation W43133674592 @default.
• W4313367459 hasOpenAccess W4313367459 @default.
• W4313367459 hasPrimaryLocation W43133674591 @default.
• W4313367459 hasRelatedWork W2017777908 @default.
• W4313367459 hasRelatedWork W2057145238 @default.
• W4313367459 hasRelatedWork W2913605136 @default.
• W4313367459 hasRelatedWork W2944062320 @default.
• W4313367459 hasRelatedWork W2980818602 @default.
• W4313367459 hasRelatedWork W3101122293 @default.
• W4313367459 hasRelatedWork W3130117825 @default.
• W4313367459 hasRelatedWork W4200540228 @default.
• W4313367459 hasRelatedWork W4220819708 @default.
• W4313367459 hasRelatedWork W4381136985 @default.
• W4313367459 hasVolume "55" @default.
• W4313367459 isParatext "false" @default.
• W4313367459 isRetracted "false" @default.
• W4313367459 workType "article" @default.