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• W4313367792 endingPage "115215" @default.
• W4313367792 startingPage "115215" @default.
• W4313367792 abstract "The sensitivity and specificity of Golgi glycoprotein 73 (GP73) are very important for early diagnosis of hepatocellular carcinoma. Herein, we constructed a new-fashioned fluorescent aptamer sensor for GP73 determination based on nitrogen-doped graphene quantum dots (N-GQDS) and molybdenum disulfide (MoS2) nanosheets. N-GQDs with high fluorescence intensity and good stability were screened out, and GP73 aptamer (GP73Apt) is labeled with N-GQDs to form the N-GQDs-GP73Apt fluorescence probe. MoS2 nanosheets can quench the fluorescence of N-GQDs-GP73Apt owing to fluorescence resonance energy transfer mechanisms. After introducing GP73 into the biosensing system, the N-GQDs-GP73Apt specifically bound with GP73 to form the deployable structures, making N-GQDs-GP73Apt far away from MoS2, blocking the fluorescence energy transfer process, and restoring the fluorescence of N-GQDs-GP73Apt. When the GP73 concentration was in the extent of 2.5 ng/mL∼100 ng/mL, the relative fluorescence recovery is linearly relevant to the concentration of GP73, and the limit of detection (LOD) was 1.29 ng/mL (S/N = 3). Moreover in the application of actual serum sample detection, the recovery was range 98.85∼100.55 %. The fluorescent aptamer sensor can rapidly detect and analyze the serum marker GP73 with the characteristics of low-cost, high sensitivity, good specificity and recovery." @default.
• W4313367792 created "2023-01-06" @default.
• W4313367792 creator A5029226448 @default.
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• W4313367792 date "2023-02-01" @default.
• W4313367792 modified "2023-09-23" @default.
• W4313367792 title "Fluorescent turn-on aptamer sensor for sensitive and reliable detection of Golgi glycoprotein 73 based on nitrogen-doped graphene quantum dots and molybdenum disulfide nanosheets" @default.
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• W4313367792 doi "https://doi.org/10.1016/j.jpba.2022.115215" @default.
• W4313367792 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36586381" @default.
• W4313367792 hasPublicationYear "2023" @default.
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