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- W4313368631 abstract "Abstract The reliance on flow cytometry for phenotypical and functional analyses of immune components in both research and clinical laboratories has increased the need flow cytometric assay standardization. Dried-down reagent cocktails pre-optimized with regards to fluorophore choice for each specificity as well as concentration of each conjugate can improve standardization. Use of a pre-optimized dried-down cocktail provides an efficient assay workflow which can minimize operator error in addition to offering enhanced reagent stability and reduced variability across instruments and sites. We developed a dried-down reagent cocktail for the identification and characterization of CD4+ and CD8+ T-cell memory and naïve subsets, including stem cell memory populations. The BD Horizon™ Dri Memory T cell panel contains optimized antibody-fluorophore conjugates against CD3, CD4, CD8, CD45RA, CD197, CD27 and CD95. Additional markers can be accommodated as drop-ins for a more comprehensive analysis. This cocktail was optimized for stain index in all channels of a red, blue, violet laser flow cytometer (BD FACSLyric™) in liquid format, then converted to dry format. The performance of the dried-down cocktail, with regards to MFI and % positivity of T-cell subsets, is comparable to that of its optimized liquid equivalents. Additionally, results are consistent across FACSLyric instruments and similarly when acquired on a BD LSRFortessa™. The comparable performance of the dried-down cocktail tube, along with the advantages offered by using a dried reagent cocktail, and the convenience of adding liquid drop-in reagents, reveal the BD Horizon Dri Memory T cell panel tube to be a valuable option for memory T-cell flow cytometry analysis." @default.
- W4313368631 created "2023-01-06" @default.
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- W4313368631 date "2020-05-01" @default.
- W4313368631 modified "2023-09-27" @default.
- W4313368631 title "A Performance-Optimized Multicolor Flow Cytometry Dried-Reagent Cocktail for Memory T-Cell Analysis" @default.
- W4313368631 doi "https://doi.org/10.4049/jimmunol.204.supp.159.51" @default.
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