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- W4313368964 abstract "Abstract We previously reported that the artificially synthesized and immunologically active lymphoid tissues (aLTs) could be stably constructed in mice by implantation of lymphoid stromal cell-embedded biocompatible scaffold (collagen sponge) as well as by applying the chemokine/lymphokine-trapped gel-embedded scaffold. The aLTs possessed the structures and functions resemble to the secondary/tertiary lymphoid organ. The antigen-specific immune response was effectively induced in the aLTs. In the present study, we have aimed to generate the aLTs composed of human lymphoid cells and human stromal cells expressing LTbR, VCAM-1, ICAM-1 and several lymphoid chemokines. The spheroids were formed as the scaffolds from the stromal cells. Then, the stromal cell spheroids were transplanted into renal subcapsular space of the immunodeficient mice together with human PBMCs absorbed in the collagen sponges. After 3 weeks, the secondary lymph node-like three-dimensional organoids (LTOs) containing clusters of the human T and B cells and the scattered human DC cells were stably formed. Next, the human LTOs were constructed by using the PBMCs prepared from healthy donors who had received once the vaccination of varicella zoster virus (VZV). The immunodeficient mice carrying human LTOs were immunized with inactivated VZV. The GC-like B cell proliferation and the production of the anti-VZV specific antibody as well as human IFNg was remarkedly induced in the LTOs. These data indicate that the immunologically functional human LTOs could be generated by applying human PBMCs with the appropriate human lymphoid stromal cells. Currently, induction of immune responses against various protein antigens as well as human cancers is under investigation." @default.
- W4313368964 created "2023-01-06" @default.
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- W4313368964 date "2020-05-01" @default.
- W4313368964 modified "2023-09-27" @default.
- W4313368964 title "Formation of human lymphoid organoids and their immunological function" @default.
- W4313368964 doi "https://doi.org/10.4049/jimmunol.204.supp.145.49" @default.
- W4313368964 hasPublicationYear "2020" @default.
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