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• W4313369013 endingPage "151992" @default.
• W4313369013 startingPage "151992" @default.
• W4313369013 abstract "Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin.Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffin-embedded. Then, approximately 100 serial sections of 4 µm thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples.From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples.All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used." @default.
• W4313369013 created "2023-01-06" @default.
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• W4313369013 date "2023-01-01" @default.
• W4313369013 modified "2023-09-27" @default.
• W4313369013 title "A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study" @default.
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• W4313369013 doi "https://doi.org/10.1016/j.acthis.2022.151992" @default.
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