Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313369042> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4313369042 endingPage "159.49" @default.
- W4313369042 startingPage "159.49" @default.
- W4313369042 abstract "Abstract We implemented the BD Rhapsody Single-Cell Analysis System to assess cellular heterogeneity present in twins by capturing and sequencing over 40000 single cells across 10 samples from 5 twins patients with asthma or without asthma using oligos-conjugated antibodies and the whole blood immune targeted gene panel that utilizes multiplex PCR for detection of over 500 genes for immune cells subtyping, immunophenotyping, cell-type identification and markers correlated with stimulations. A combinatorial library of beads bearing cell- and molecular-barcoding capture probes is used to uniquely label transcripts and reconstruct the digital gene expression profile of thousands of individual cells in a single experiment without the need for robotics or automation. We demonstrate the ability of the BD Rhapsody targeted panel to distinguish six different cell types and 18 distinct gene-expression biomarkers, including CLEC4E, CSF2, IL1RN, TPSAB1, CCL19, CCL22, CXCL2, LAMP3, SPP1, and VMO etc.. The HLA-DRA, HLA-DQB1, SPP1, and IL1RN had allelic polymorphism related to asthma. The BD Rhapsody targeted panel yields more sequencing depth with much fewer (<2%) sequencing reads per cell. The measurement of specific proteins and transcripts in individual cells is critical for understanding the role of cellular diversity in development, health, and disease." @default.
- W4313369042 created "2023-01-06" @default.
- W4313369042 creator A5017158014 @default.
- W4313369042 creator A5028760615 @default.
- W4313369042 creator A5032321375 @default.
- W4313369042 creator A5035178678 @default.
- W4313369042 creator A5038093922 @default.
- W4313369042 creator A5050399447 @default.
- W4313369042 creator A5068399737 @default.
- W4313369042 creator A5080923237 @default.
- W4313369042 date "2020-05-01" @default.
- W4313369042 modified "2023-09-27" @default.
- W4313369042 title "Protein- and sequencing-based massively parallel single-cell approaches to immune profiling asthma-specific cell subtypes and related gene expression biomarkers" @default.
- W4313369042 doi "https://doi.org/10.4049/jimmunol.204.supp.159.49" @default.
- W4313369042 hasPublicationYear "2020" @default.
- W4313369042 type Work @default.
- W4313369042 citedByCount "0" @default.
- W4313369042 crossrefType "journal-article" @default.
- W4313369042 hasAuthorship W4313369042A5017158014 @default.
- W4313369042 hasAuthorship W4313369042A5028760615 @default.
- W4313369042 hasAuthorship W4313369042A5032321375 @default.
- W4313369042 hasAuthorship W4313369042A5035178678 @default.
- W4313369042 hasAuthorship W4313369042A5038093922 @default.
- W4313369042 hasAuthorship W4313369042A5050399447 @default.
- W4313369042 hasAuthorship W4313369042A5068399737 @default.
- W4313369042 hasAuthorship W4313369042A5080923237 @default.
- W4313369042 hasConcept C104317684 @default.
- W4313369042 hasConcept C150194340 @default.
- W4313369042 hasConcept C18431079 @default.
- W4313369042 hasConcept C203014093 @default.
- W4313369042 hasConcept C2781188995 @default.
- W4313369042 hasConcept C54355233 @default.
- W4313369042 hasConcept C70721500 @default.
- W4313369042 hasConcept C86803240 @default.
- W4313369042 hasConcept C8891405 @default.
- W4313369042 hasConceptScore W4313369042C104317684 @default.
- W4313369042 hasConceptScore W4313369042C150194340 @default.
- W4313369042 hasConceptScore W4313369042C18431079 @default.
- W4313369042 hasConceptScore W4313369042C203014093 @default.
- W4313369042 hasConceptScore W4313369042C2781188995 @default.
- W4313369042 hasConceptScore W4313369042C54355233 @default.
- W4313369042 hasConceptScore W4313369042C70721500 @default.
- W4313369042 hasConceptScore W4313369042C86803240 @default.
- W4313369042 hasConceptScore W4313369042C8891405 @default.
- W4313369042 hasIssue "1_Supplement" @default.
- W4313369042 hasLocation W43133690421 @default.
- W4313369042 hasOpenAccess W4313369042 @default.
- W4313369042 hasPrimaryLocation W43133690421 @default.
- W4313369042 hasRelatedWork W2009966535 @default.
- W4313369042 hasRelatedWork W2052655934 @default.
- W4313369042 hasRelatedWork W2053053668 @default.
- W4313369042 hasRelatedWork W2111172999 @default.
- W4313369042 hasRelatedWork W2114454856 @default.
- W4313369042 hasRelatedWork W2348296989 @default.
- W4313369042 hasRelatedWork W2365592492 @default.
- W4313369042 hasRelatedWork W2376837706 @default.
- W4313369042 hasRelatedWork W3012091774 @default.
- W4313369042 hasRelatedWork W3032874291 @default.
- W4313369042 hasVolume "204" @default.
- W4313369042 isParatext "false" @default.
- W4313369042 isRetracted "false" @default.
- W4313369042 workType "article" @default.