Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313369047> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4313369047 endingPage "151.6" @default.
- W4313369047 startingPage "151.6" @default.
- W4313369047 abstract "Abstract We have previously described a post-transcriptional pathway of induced mRNA stability that occurs at late times of T cell activation and is engaged by the binding of polypyrimidine tract-binding protein (PTBP1) complex to the 3′ UTR of the CD40L transcript. We recently explored the in vivo role of this pathway by generating a genetically modified mouse (CD40LΔ5) bearing a deletion of the PTBP1 stability element. We found that the CD40LΔ5 mice display a dysfunctional germinal center (GC) response including significantly decreased levels of isotype switched antibodies, antibody-secreting cells, memory B cells and GL7+ GC B cells. To extend our earlier findings we measured affinity maturation of antibodies in response to the hapten NP and found a loss of high affinity binding that corresponded to reduced numbers of targeted VH mutations known to be associated with high affinity binding. However, there were no discernible differences in the overall number of mutations across the VH segment, suggesting that the CD40LΔ5 pathway may have a greater effect on the selection and/or expansion of high-affinity B cells rather than on the somatic hypermutation (SHM) in general. To identify how the CD40L mRNA stability pathway impacted B cells during an immune response, CD19+ B cells from immunized mice were transcriptionally profiled using RNAseq. Expression of genes associated with cell survival and proliferation were found to be down-regulated and apoptotic genes up-regulated in CD40LΔ5 B cells compared to WT cells. Together these finding are consistent with the Δ5 stability element playing a critical role in the proliferation and cell survival of high affinity GC B cells through a pathway of enhanced expression of CD40L in CD4 T cells." @default.
- W4313369047 created "2023-01-06" @default.
- W4313369047 creator A5004911213 @default.
- W4313369047 creator A5019571545 @default.
- W4313369047 creator A5028506156 @default.
- W4313369047 creator A5037061468 @default.
- W4313369047 creator A5069464227 @default.
- W4313369047 date "2020-05-01" @default.
- W4313369047 modified "2023-10-18" @default.
- W4313369047 title "Activation-dependent post-transcriptional regulation of CD40L mediates B cell development and survival in germinal centers" @default.
- W4313369047 doi "https://doi.org/10.4049/jimmunol.204.supp.151.6" @default.
- W4313369047 hasPublicationYear "2020" @default.
- W4313369047 type Work @default.
- W4313369047 citedByCount "0" @default.
- W4313369047 crossrefType "journal-article" @default.
- W4313369047 hasAuthorship W4313369047A5004911213 @default.
- W4313369047 hasAuthorship W4313369047A5019571545 @default.
- W4313369047 hasAuthorship W4313369047A5028506156 @default.
- W4313369047 hasAuthorship W4313369047A5037061468 @default.
- W4313369047 hasAuthorship W4313369047A5069464227 @default.
- W4313369047 hasConcept C128526571 @default.
- W4313369047 hasConcept C137756551 @default.
- W4313369047 hasConcept C153911025 @default.
- W4313369047 hasConcept C154317977 @default.
- W4313369047 hasConcept C159654299 @default.
- W4313369047 hasConcept C202751555 @default.
- W4313369047 hasConcept C203014093 @default.
- W4313369047 hasConcept C2778453870 @default.
- W4313369047 hasConcept C2778957590 @default.
- W4313369047 hasConcept C2781377902 @default.
- W4313369047 hasConcept C39347974 @default.
- W4313369047 hasConcept C54355233 @default.
- W4313369047 hasConcept C553184892 @default.
- W4313369047 hasConcept C86803240 @default.
- W4313369047 hasConcept C95444343 @default.
- W4313369047 hasConcept C96926380 @default.
- W4313369047 hasConceptScore W4313369047C128526571 @default.
- W4313369047 hasConceptScore W4313369047C137756551 @default.
- W4313369047 hasConceptScore W4313369047C153911025 @default.
- W4313369047 hasConceptScore W4313369047C154317977 @default.
- W4313369047 hasConceptScore W4313369047C159654299 @default.
- W4313369047 hasConceptScore W4313369047C202751555 @default.
- W4313369047 hasConceptScore W4313369047C203014093 @default.
- W4313369047 hasConceptScore W4313369047C2778453870 @default.
- W4313369047 hasConceptScore W4313369047C2778957590 @default.
- W4313369047 hasConceptScore W4313369047C2781377902 @default.
- W4313369047 hasConceptScore W4313369047C39347974 @default.
- W4313369047 hasConceptScore W4313369047C54355233 @default.
- W4313369047 hasConceptScore W4313369047C553184892 @default.
- W4313369047 hasConceptScore W4313369047C86803240 @default.
- W4313369047 hasConceptScore W4313369047C95444343 @default.
- W4313369047 hasConceptScore W4313369047C96926380 @default.
- W4313369047 hasIssue "1_Supplement" @default.
- W4313369047 hasLocation W43133690471 @default.
- W4313369047 hasOpenAccess W4313369047 @default.
- W4313369047 hasPrimaryLocation W43133690471 @default.
- W4313369047 hasRelatedWork W1980907725 @default.
- W4313369047 hasRelatedWork W2065906324 @default.
- W4313369047 hasRelatedWork W2088870397 @default.
- W4313369047 hasRelatedWork W2112061682 @default.
- W4313369047 hasRelatedWork W2136877952 @default.
- W4313369047 hasRelatedWork W3168793884 @default.
- W4313369047 hasRelatedWork W3171487727 @default.
- W4313369047 hasRelatedWork W4200628384 @default.
- W4313369047 hasRelatedWork W4313369047 @default.
- W4313369047 hasRelatedWork W4319432065 @default.
- W4313369047 hasVolume "204" @default.
- W4313369047 isParatext "false" @default.
- W4313369047 isRetracted "false" @default.
- W4313369047 workType "article" @default.