Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313369112> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W4313369112 endingPage "156.26" @default.
- W4313369112 startingPage "156.26" @default.
- W4313369112 abstract "Abstract Rationale Candida albicans (CA) is ubiquitous and individuals are often immunized against it. However, skin testing reactivity with CA-antigens (CAAg) have been variable during latent tuberculosis infection (LTBI) screening. We hypothesized that the use of a CAAg in an ELISPOT assay would provide a positive control to study tuberculosis (TB)-antigen specific immune responses. Objective To test an ELISPOT with TB-antigens and control conditions, including a purified protein derivative of CA, CAAg, that differentiates subjects with LTBI from unexposed subjects. Methods An ELISPOT that detects interferon-γ from PBMCs (2×105 cells) after 2-day incubation with TB antigens, controls, including CAAg, and co-stimulatory antibodies. We studied two independent cohorts of subjects with LTBI diagnosis based on TST and IGRA results per clinical guidelines. Results One-hundred thirty-nine subjects were studied in 2010–12, including 27 patients with LTBI and 21 IGRA(−) unexposed subjects. ELISPOT with TB-antigens but also CAAg - nil (78 [21 – 182] vs. 9 [1 – 50] cfu) differentiated LTBI subjects from unexposed ones (P<0.005). A second cohort of 76 subjects were studied in 2015–17, including 14 patients with LTBI and 24 unexposed controls, which showed similar statistical results. Positive control (SEAB/antiCD3) did not differentiate these study groups. ELISPOT results with CAAg correlated with results of ELISPOT with TB antigens in all subjects (P<0.001), and in the LTBI and unexposed groups independently. Conclusion An ELISPOT assay that includes CAAg revealed immune reactivity that is prevalent in LTBI. The immune subsets that react to both CA and mycobacterial antigens are unclear, but may be relevant for both TB and candida immunity." @default.
- W4313369112 created "2023-01-06" @default.
- W4313369112 creator A5004579335 @default.
- W4313369112 creator A5041817739 @default.
- W4313369112 creator A5063291224 @default.
- W4313369112 creator A5065454745 @default.
- W4313369112 creator A5081212705 @default.
- W4313369112 date "2020-05-01" @default.
- W4313369112 modified "2023-09-27" @default.
- W4313369112 title "Immune reactivity to tuberculosis and candida antigens in subjects with latent tuberculosis infection and unexposed subjects: A validation cohort study" @default.
- W4313369112 doi "https://doi.org/10.4049/jimmunol.204.supp.156.26" @default.
- W4313369112 hasPublicationYear "2020" @default.
- W4313369112 type Work @default.
- W4313369112 citedByCount "0" @default.
- W4313369112 crossrefType "journal-article" @default.
- W4313369112 hasAuthorship W4313369112A5004579335 @default.
- W4313369112 hasAuthorship W4313369112A5041817739 @default.
- W4313369112 hasAuthorship W4313369112A5063291224 @default.
- W4313369112 hasAuthorship W4313369112A5065454745 @default.
- W4313369112 hasAuthorship W4313369112A5081212705 @default.
- W4313369112 hasConcept C126322002 @default.
- W4313369112 hasConcept C142724271 @default.
- W4313369112 hasConcept C147483822 @default.
- W4313369112 hasConcept C203014093 @default.
- W4313369112 hasConcept C2776090121 @default.
- W4313369112 hasConcept C2776247216 @default.
- W4313369112 hasConcept C2777975735 @default.
- W4313369112 hasConcept C2779053233 @default.
- W4313369112 hasConcept C2779806340 @default.
- W4313369112 hasConcept C2781069245 @default.
- W4313369112 hasConcept C71924100 @default.
- W4313369112 hasConcept C72563966 @default.
- W4313369112 hasConcept C8891405 @default.
- W4313369112 hasConceptScore W4313369112C126322002 @default.
- W4313369112 hasConceptScore W4313369112C142724271 @default.
- W4313369112 hasConceptScore W4313369112C147483822 @default.
- W4313369112 hasConceptScore W4313369112C203014093 @default.
- W4313369112 hasConceptScore W4313369112C2776090121 @default.
- W4313369112 hasConceptScore W4313369112C2776247216 @default.
- W4313369112 hasConceptScore W4313369112C2777975735 @default.
- W4313369112 hasConceptScore W4313369112C2779053233 @default.
- W4313369112 hasConceptScore W4313369112C2779806340 @default.
- W4313369112 hasConceptScore W4313369112C2781069245 @default.
- W4313369112 hasConceptScore W4313369112C71924100 @default.
- W4313369112 hasConceptScore W4313369112C72563966 @default.
- W4313369112 hasConceptScore W4313369112C8891405 @default.
- W4313369112 hasIssue "1_Supplement" @default.
- W4313369112 hasLocation W43133691121 @default.
- W4313369112 hasOpenAccess W4313369112 @default.
- W4313369112 hasPrimaryLocation W43133691121 @default.
- W4313369112 hasRelatedWork W1968263951 @default.
- W4313369112 hasRelatedWork W1969918677 @default.
- W4313369112 hasRelatedWork W2025404538 @default.
- W4313369112 hasRelatedWork W2058168006 @default.
- W4313369112 hasRelatedWork W2125665652 @default.
- W4313369112 hasRelatedWork W2132180171 @default.
- W4313369112 hasRelatedWork W2189046464 @default.
- W4313369112 hasRelatedWork W2278154581 @default.
- W4313369112 hasRelatedWork W2604543294 @default.
- W4313369112 hasRelatedWork W4281487762 @default.
- W4313369112 hasVolume "204" @default.
- W4313369112 isParatext "false" @default.
- W4313369112 isRetracted "false" @default.
- W4313369112 workType "article" @default.