Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313369214> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4313369214 endingPage "145.38" @default.
- W4313369214 startingPage "145.38" @default.
- W4313369214 abstract "Abstract Adipose tissue (AT) regulatory T Cells (Tregs) play a key role in regulating inflammatory and metabolic responses. Obesity is associated with a decrease in AT Tregs as well as an increase in tissue inflammation and systemic insulin resistance in both humans and mice. We hypothesize that, in addition to the decrease in AT Treg number, there is a dysfunctional Treg phenotype caused by the increased inflammatory state within obese AT. We isolated Tregs from AT and peripheral blood(PB) from lean and obese individuals. Real-time PCR analysis was used to measure the expression of several known markers for T cell exhaustion. Protein expression of each marker was validated using FACS analysis. When compared to PB, there was a significant increase in PD-1 expression on AT Tregs. PD-1 and TIGIT were increased in Tregs isolated from obese vs. lean individuals, while CD127 expression was reduced. To determine if PD-1 expression correlates with Treg dysfunction, isolated AT and PB Tregs were added to CFSE-labeled PBMC. After 4 days culture, PB Tregs significantly inhibited proliferation but there was less suppression by AT Tregs. RNAseq analyses of PD-1 high vs. PD-1 negative AT and PB Tregs revealed a unique expression phenotype within AT Tregs. AT PD-1 high Tregs express altered metabolic and inflammatory pathways promoting loss of Tregs and increased inflammation. These data suggest that Treg exhaustion may explain the decreased Treg abundance in AT of obese individuals, which has important metabolic implications. (Partially supported by Foods for Health, a focus area of the Discovery Themes Initiative at The Ohio State University)" @default.
- W4313369214 created "2023-01-06" @default.
- W4313369214 creator A5010603589 @default.
- W4313369214 creator A5031105971 @default.
- W4313369214 creator A5037884146 @default.
- W4313369214 creator A5072110516 @default.
- W4313369214 creator A5074250515 @default.
- W4313369214 creator A5074408622 @default.
- W4313369214 date "2020-05-01" @default.
- W4313369214 modified "2023-10-14" @default.
- W4313369214 title "Obesity contributes to a dysfunctional regulatory T Cell phenotype within adipose tissue" @default.
- W4313369214 doi "https://doi.org/10.4049/jimmunol.204.supp.145.38" @default.
- W4313369214 hasPublicationYear "2020" @default.
- W4313369214 type Work @default.
- W4313369214 citedByCount "4" @default.
- W4313369214 countsByYear W43133692142021 @default.
- W4313369214 countsByYear W43133692142022 @default.
- W4313369214 crossrefType "journal-article" @default.
- W4313369214 hasAuthorship W4313369214A5010603589 @default.
- W4313369214 hasAuthorship W4313369214A5031105971 @default.
- W4313369214 hasAuthorship W4313369214A5037884146 @default.
- W4313369214 hasAuthorship W4313369214A5072110516 @default.
- W4313369214 hasAuthorship W4313369214A5074250515 @default.
- W4313369214 hasAuthorship W4313369214A5074408622 @default.
- W4313369214 hasConcept C104317684 @default.
- W4313369214 hasConcept C123982805 @default.
- W4313369214 hasConcept C126322002 @default.
- W4313369214 hasConcept C127716648 @default.
- W4313369214 hasConcept C134018914 @default.
- W4313369214 hasConcept C137061746 @default.
- W4313369214 hasConcept C171089720 @default.
- W4313369214 hasConcept C202751555 @default.
- W4313369214 hasConcept C203014093 @default.
- W4313369214 hasConcept C2776090121 @default.
- W4313369214 hasConcept C2776914184 @default.
- W4313369214 hasConcept C2778296632 @default.
- W4313369214 hasConcept C2779727006 @default.
- W4313369214 hasConcept C54355233 @default.
- W4313369214 hasConcept C71924100 @default.
- W4313369214 hasConcept C79484868 @default.
- W4313369214 hasConcept C86803240 @default.
- W4313369214 hasConcept C8891405 @default.
- W4313369214 hasConceptScore W4313369214C104317684 @default.
- W4313369214 hasConceptScore W4313369214C123982805 @default.
- W4313369214 hasConceptScore W4313369214C126322002 @default.
- W4313369214 hasConceptScore W4313369214C127716648 @default.
- W4313369214 hasConceptScore W4313369214C134018914 @default.
- W4313369214 hasConceptScore W4313369214C137061746 @default.
- W4313369214 hasConceptScore W4313369214C171089720 @default.
- W4313369214 hasConceptScore W4313369214C202751555 @default.
- W4313369214 hasConceptScore W4313369214C203014093 @default.
- W4313369214 hasConceptScore W4313369214C2776090121 @default.
- W4313369214 hasConceptScore W4313369214C2776914184 @default.
- W4313369214 hasConceptScore W4313369214C2778296632 @default.
- W4313369214 hasConceptScore W4313369214C2779727006 @default.
- W4313369214 hasConceptScore W4313369214C54355233 @default.
- W4313369214 hasConceptScore W4313369214C71924100 @default.
- W4313369214 hasConceptScore W4313369214C79484868 @default.
- W4313369214 hasConceptScore W4313369214C86803240 @default.
- W4313369214 hasConceptScore W4313369214C8891405 @default.
- W4313369214 hasIssue "1_Supplement" @default.
- W4313369214 hasLocation W43133692141 @default.
- W4313369214 hasOpenAccess W4313369214 @default.
- W4313369214 hasPrimaryLocation W43133692141 @default.
- W4313369214 hasRelatedWork W137722765 @default.
- W4313369214 hasRelatedWork W1518295571 @default.
- W4313369214 hasRelatedWork W154621678 @default.
- W4313369214 hasRelatedWork W1997714882 @default.
- W4313369214 hasRelatedWork W2015386355 @default.
- W4313369214 hasRelatedWork W2210159767 @default.
- W4313369214 hasRelatedWork W2271696469 @default.
- W4313369214 hasRelatedWork W2382901196 @default.
- W4313369214 hasRelatedWork W2750464235 @default.
- W4313369214 hasRelatedWork W2128850852 @default.
- W4313369214 hasVolume "204" @default.
- W4313369214 isParatext "false" @default.
- W4313369214 isRetracted "false" @default.
- W4313369214 workType "article" @default.