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• W4313371594 endingPage "8848" @default.
• W4313371594 startingPage "8839" @default.
• W4313371594 abstract "The phenotype of substantia nigra (SN) neurons in homozygous weaver ( wv/wv ) mice was studied by combining patch-clamp and single-cell RT-multiplex PCR techniques in midbrain slices of 14-d-old mice. In contrast to GABAergic SN neurons, which were unaffected in homozygous weaver mice ( wv/wv ), dopaminergic SN neurons possessed a dramatically altered phenotype with a depolarized membrane potential and complete loss of spontaneous pacemaker activity. The gain-of-function phenotype was mediated by a large, nonselective membrane conductance exclusively present in ( wv/wv ) dopaminergic SN neurons. This constitutively activated conductance displayed a sensitivity to external QX-314 (IC 50 = 10.6 μ m ) very similar to that of heterologously expressed wv Girk2 channels and was not further activated by G-protein stimulation. Single-cell Girk1–4 expression profiling suggested that homomeric Girk2 channels were present in most dopaminergic SN neurons, whereas Girk2 was always coexpressed with other Girk family members in GABAergic SN neurons. Surprisingly, acute QX-314 inhibition of wv Girk2 channels did not induce wild-type-like pacemaker activity but instead caused membrane hyperpolarization. Additional application of a blocker of ATP-sensitive potassium channels (100 μ m tolbutamide) induced wild-type-like pacemaker activity. We conclude that the gain-of-function weaver phenotype of dopaminergic substantia nigra neurons is mediated by coactivation of wv Girk2 and SUR1/Kir6.2-mediated ATP-sensitive K + channels. We also show that in contrast to wild-type neurons, all ( wv/wv ) dopaminergic SN neurons expressed calbindin, a calcium-binding protein that marks dopaminergic SN neurons resistant to neurodegeneration. The identification of two ion channels that in concert determine the weaver phenotype of surviving calbindin-positive dopaminergic SN neurons will help to understand the molecular mechanisms of selective neurodegeneration of dopaminergic SN neurons in the weaver mouse and might be important in Parkinson’s disease." @default.
• W4313371594 created "2023-01-06" @default.
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• W4313371594 date "1999-10-15" @default.
• W4313371594 modified "2023-09-28" @default.
• W4313371594 title "The<i>weaver</i>Mouse<i>gain-of-function</i>Phenotype of Dopaminergic Midbrain Neurons Is Determined by Coactivation of<i>wv</i>Girk2 and K-ATP Channels" @default.
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• W4313371594 doi "https://doi.org/10.1523/jneurosci.19-20-08839.1999" @default.
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