Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313373210> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W4313373210 endingPage "86.4" @default.
- W4313373210 startingPage "86.4" @default.
- W4313373210 abstract "Abstract For the last 20 years, intravital confocal and two-photon microscopy have been powerful tools to explore dynamic immune cell behavior in mouse lymph node. However, they can only image ~100 and ~300 μm depth respectively while a peripheral lymph node of adult mouse has 600 – 1000 μm thickness. Here, we visualized whole thickness of adult mouse popliteal lymph node with intravital three-photon microscopy. Three-photon excitation significantly improved a signal-to-background ratio compared to two-photon excitation even at the same excitation wavelength. The capability to image the full depth enabled to achieve the 3D volume of entire lymph node vasculature in vivo by tiling multiple z-stack images. We observed DsRed-expressing lymphocyte migration in LYVE1+ lymphatic sinus at 600 μm depth of lymph node where conventional two-photon microscopy was normally inaccessible. In addition, we demonstrated the capability multi-color imaging by using Cγ1-Confetti mice of which each germinal center B cells stochastically expresses one of 4 different fluorescent proteins (CFP, GFP, YFP and RFP). Intravital three-photon microscopy has the potential to shed light on unknown immune cell behavior in deeper regions of the lymph node in vivo just as two-photon microscopy did during the last 20 years at the shallower depth." @default.
- W4313373210 created "2023-01-06" @default.
- W4313373210 creator A5012115309 @default.
- W4313373210 creator A5019770989 @default.
- W4313373210 creator A5037793982 @default.
- W4313373210 creator A5046510603 @default.
- W4313373210 creator A5057491420 @default.
- W4313373210 creator A5062542913 @default.
- W4313373210 creator A5064655594 @default.
- W4313373210 creator A5076186884 @default.
- W4313373210 creator A5090690804 @default.
- W4313373210 date "2020-05-01" @default.
- W4313373210 modified "2023-09-27" @default.
- W4313373210 title "Intravital three-photon microscopy of entire mouse lymph node" @default.
- W4313373210 doi "https://doi.org/10.4049/jimmunol.204.supp.86.4" @default.
- W4313373210 hasPublicationYear "2020" @default.
- W4313373210 type Work @default.
- W4313373210 citedByCount "0" @default.
- W4313373210 crossrefType "journal-article" @default.
- W4313373210 hasAuthorship W4313373210A5012115309 @default.
- W4313373210 hasAuthorship W4313373210A5019770989 @default.
- W4313373210 hasAuthorship W4313373210A5037793982 @default.
- W4313373210 hasAuthorship W4313373210A5046510603 @default.
- W4313373210 hasAuthorship W4313373210A5057491420 @default.
- W4313373210 hasAuthorship W4313373210A5062542913 @default.
- W4313373210 hasAuthorship W4313373210A5064655594 @default.
- W4313373210 hasAuthorship W4313373210A5076186884 @default.
- W4313373210 hasAuthorship W4313373210A5090690804 @default.
- W4313373210 hasConcept C120665830 @default.
- W4313373210 hasConcept C121332964 @default.
- W4313373210 hasConcept C12554922 @default.
- W4313373210 hasConcept C142724271 @default.
- W4313373210 hasConcept C147080431 @default.
- W4313373210 hasConcept C150903083 @default.
- W4313373210 hasConcept C159654299 @default.
- W4313373210 hasConcept C169274487 @default.
- W4313373210 hasConcept C181152851 @default.
- W4313373210 hasConcept C192562407 @default.
- W4313373210 hasConcept C203014093 @default.
- W4313373210 hasConcept C207001950 @default.
- W4313373210 hasConcept C2778453870 @default.
- W4313373210 hasConcept C2779178360 @default.
- W4313373210 hasConcept C2779720271 @default.
- W4313373210 hasConcept C2780849966 @default.
- W4313373210 hasConcept C2859252 @default.
- W4313373210 hasConcept C47785574 @default.
- W4313373210 hasConcept C62520636 @default.
- W4313373210 hasConcept C62611344 @default.
- W4313373210 hasConcept C71924100 @default.
- W4313373210 hasConcept C86803240 @default.
- W4313373210 hasConcept C91881484 @default.
- W4313373210 hasConcept C95444343 @default.
- W4313373210 hasConcept C96926380 @default.
- W4313373210 hasConceptScore W4313373210C120665830 @default.
- W4313373210 hasConceptScore W4313373210C121332964 @default.
- W4313373210 hasConceptScore W4313373210C12554922 @default.
- W4313373210 hasConceptScore W4313373210C142724271 @default.
- W4313373210 hasConceptScore W4313373210C147080431 @default.
- W4313373210 hasConceptScore W4313373210C150903083 @default.
- W4313373210 hasConceptScore W4313373210C159654299 @default.
- W4313373210 hasConceptScore W4313373210C169274487 @default.
- W4313373210 hasConceptScore W4313373210C181152851 @default.
- W4313373210 hasConceptScore W4313373210C192562407 @default.
- W4313373210 hasConceptScore W4313373210C203014093 @default.
- W4313373210 hasConceptScore W4313373210C207001950 @default.
- W4313373210 hasConceptScore W4313373210C2778453870 @default.
- W4313373210 hasConceptScore W4313373210C2779178360 @default.
- W4313373210 hasConceptScore W4313373210C2779720271 @default.
- W4313373210 hasConceptScore W4313373210C2780849966 @default.
- W4313373210 hasConceptScore W4313373210C2859252 @default.
- W4313373210 hasConceptScore W4313373210C47785574 @default.
- W4313373210 hasConceptScore W4313373210C62520636 @default.
- W4313373210 hasConceptScore W4313373210C62611344 @default.
- W4313373210 hasConceptScore W4313373210C71924100 @default.
- W4313373210 hasConceptScore W4313373210C86803240 @default.
- W4313373210 hasConceptScore W4313373210C91881484 @default.
- W4313373210 hasConceptScore W4313373210C95444343 @default.
- W4313373210 hasConceptScore W4313373210C96926380 @default.
- W4313373210 hasIssue "1_Supplement" @default.
- W4313373210 hasLocation W43133732101 @default.
- W4313373210 hasOpenAccess W4313373210 @default.
- W4313373210 hasPrimaryLocation W43133732101 @default.
- W4313373210 hasRelatedWork W1977388470 @default.
- W4313373210 hasRelatedWork W2017379512 @default.
- W4313373210 hasRelatedWork W2017909074 @default.
- W4313373210 hasRelatedWork W2056698445 @default.
- W4313373210 hasRelatedWork W2267402106 @default.
- W4313373210 hasRelatedWork W4214912729 @default.
- W4313373210 hasRelatedWork W4285387429 @default.
- W4313373210 hasRelatedWork W4313373210 @default.
- W4313373210 hasRelatedWork W43671713 @default.
- W4313373210 hasRelatedWork W60383273 @default.
- W4313373210 hasVolume "204" @default.
- W4313373210 isParatext "false" @default.
- W4313373210 isRetracted "false" @default.
- W4313373210 workType "article" @default.