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- W4313373810 startingPage "63.11" @default.
- W4313373810 abstract "Abstract Tissue-resident macrophages (TRMΦ) are important immune sentinels responsible for maintaining tissue and immune homeostasis, where most develop during embryogenesis independently of long-term hematopoietic stem cells (LT-HSCs) and are maintained throughout life with minimal contribution from circulating monocytes. Recent studies have shown that, in certain conditions, infiltrating monocyte-derived (MD)MΦ can supplement TRMΦ, but remain distinguishable. However, the phenotypic and functional heterogeneity of TRMΦ versus their supplemented MDMΦ counterparts in each organ system has not been established. To address this gap, we have generated a novel dual-reporter lineage tracing model to delineate TRMΦ vs MDMΦ in vivo. We used a non-genotoxic method of HSC depletion in the Runx1Cre/eGFP mouse, which allows us to transplant LT-HSCs expressing mRFP1. These dual-reporter mice exhibit normal supplementation of MDMΦ (red) to the existing TRMΦ (green) pool. We have previously identified two subsets of peritoneal MΦ that exhibit dual ontogeny. However, an in-depth examination of the phenotypic and functional differences between these two MΦ lineages is lacking. Using our new model, we performed single-cell mRNA sequencing and high-dimensional flow cytometric analyses and reveal further heterogeneity in peritoneal MΦ across lineages, supporting the notion that infiltrating MDMΦ exhibit phenotypic divergence from TRMΦ with potential functional impact. Here, we report the generation and utility of an innovative model incorporating lineage-specific fluorescent reporters in radiation-naïve mice, an important tool for understanding phenotypic and functional diversity of MΦ with different developmental origins in vivo." @default.
- W4313373810 created "2023-01-06" @default.
- W4313373810 creator A5004350999 @default.
- W4313373810 creator A5037605645 @default.
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- W4313373810 date "2020-05-01" @default.
- W4313373810 modified "2023-09-27" @default.
- W4313373810 title "A novel dual-reporter lineage tracing model reveals phenotypic and functional heterogeneity of tissue-resident macrophages" @default.
- W4313373810 doi "https://doi.org/10.4049/jimmunol.204.supp.63.11" @default.
- W4313373810 hasPublicationYear "2020" @default.
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