Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313374633> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W4313374633 endingPage "92.44" @default.
- W4313374633 startingPage "92.44" @default.
- W4313374633 abstract "Abstract Mosquitoes are vectors of many infectious diseases that constitute a risk to human health around the globe. Arthropod-borne viruses (arboviruses) such as Dengue and Zika viruses, are mainly transmitted by Aedes mosquito species, while plasmodium is spread by members of the Anopheles genus. The vectorial capacity of mosquito is based on a complex balance between the mosquito’s immune control and evasion mechanisms by the pathogen. The Toll signalling pathway is a key component of insect innate immunity that underwent duplication in mosquitoes compared to Drosophila melanogaster (Dm). Here we produced duplicated Spz isoforms and ectodomains of Toll1A and Toll5A in order to study binding specificity and signalling in Aedes aegypti (Aa). Surprisingly, Toll1A, the closest structural homolog to DmToll, did not bind to DmSpz, AaSpz1B or AaSpz1C, while Toll5A specifically bound to Spz1C. In Aag2 cells, however, DmSpz triggered gambicin production upon proteolytic processing, in contrast to mosquito Spz isoforms, which lead to a distinct set of antimicrobial peptides. Computational pairing of Toll and Spz failed to predict isoform and species-specificity, probably due to conformational changes upon ligand binding as observed by SAXS analysis of Toll5A-Spz1C complex. This study sheds light on the molecular basis of Toll and Spz neofunctionalization in the mosquito." @default.
- W4313374633 created "2023-01-06" @default.
- W4313374633 creator A5011017653 @default.
- W4313374633 creator A5046551506 @default.
- W4313374633 creator A5057333378 @default.
- W4313374633 date "2020-05-01" @default.
- W4313374633 modified "2023-10-02" @default.
- W4313374633 title "Structural and functional divergence of duplicated mosquito Toll and Spz compared to Drosophila" @default.
- W4313374633 doi "https://doi.org/10.4049/jimmunol.204.supp.92.44" @default.
- W4313374633 hasPublicationYear "2020" @default.
- W4313374633 type Work @default.
- W4313374633 citedByCount "0" @default.
- W4313374633 crossrefType "journal-article" @default.
- W4313374633 hasAuthorship W4313374633A5011017653 @default.
- W4313374633 hasAuthorship W4313374633A5046551506 @default.
- W4313374633 hasAuthorship W4313374633A5057333378 @default.
- W4313374633 hasConcept C104317684 @default.
- W4313374633 hasConcept C136449434 @default.
- W4313374633 hasConcept C159047783 @default.
- W4313374633 hasConcept C173758957 @default.
- W4313374633 hasConcept C18903297 @default.
- W4313374633 hasConcept C2777775583 @default.
- W4313374633 hasConcept C2780104201 @default.
- W4313374633 hasConcept C2780688631 @default.
- W4313374633 hasConcept C533803919 @default.
- W4313374633 hasConcept C54355233 @default.
- W4313374633 hasConcept C86803240 @default.
- W4313374633 hasConcept C8891405 @default.
- W4313374633 hasConceptScore W4313374633C104317684 @default.
- W4313374633 hasConceptScore W4313374633C136449434 @default.
- W4313374633 hasConceptScore W4313374633C159047783 @default.
- W4313374633 hasConceptScore W4313374633C173758957 @default.
- W4313374633 hasConceptScore W4313374633C18903297 @default.
- W4313374633 hasConceptScore W4313374633C2777775583 @default.
- W4313374633 hasConceptScore W4313374633C2780104201 @default.
- W4313374633 hasConceptScore W4313374633C2780688631 @default.
- W4313374633 hasConceptScore W4313374633C533803919 @default.
- W4313374633 hasConceptScore W4313374633C54355233 @default.
- W4313374633 hasConceptScore W4313374633C86803240 @default.
- W4313374633 hasConceptScore W4313374633C8891405 @default.
- W4313374633 hasIssue "1_Supplement" @default.
- W4313374633 hasLocation W43133746331 @default.
- W4313374633 hasOpenAccess W4313374633 @default.
- W4313374633 hasPrimaryLocation W43133746331 @default.
- W4313374633 hasRelatedWork W1510648115 @default.
- W4313374633 hasRelatedWork W1994279539 @default.
- W4313374633 hasRelatedWork W2419670143 @default.
- W4313374633 hasRelatedWork W2606890618 @default.
- W4313374633 hasRelatedWork W2950693591 @default.
- W4313374633 hasRelatedWork W3177163781 @default.
- W4313374633 hasRelatedWork W3215161474 @default.
- W4313374633 hasRelatedWork W4283034103 @default.
- W4313374633 hasRelatedWork W3148426222 @default.
- W4313374633 hasRelatedWork W337062004 @default.
- W4313374633 hasVolume "204" @default.
- W4313374633 isParatext "false" @default.
- W4313374633 isRetracted "false" @default.
- W4313374633 workType "article" @default.