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- W4313376816 abstract "Summary The three known antigenic components demonstrable in foot and mouth disease infectious tissue culture fluids were isolated and examined for complement-fixing activity. Antisera containing antibody to the three antigens titered higher against the 140 S virus particles than with the 12 S protein subunit or infection-associated antigens. The difference in serum titers with the three antigens was more evident using a fixation period of 1 ½ hr at 37°C than one of 20 hr at 4°C. Advantage was taken of this fact to develop a quantitative assay for 140 S antigen. It was found that 0.231 ± 0.035 µg/ml of 140 S antigen fixed 4 of 5 50% hemolytic units of complement with a selected antiserum dilution using a fixation period of 1 ½ hr at 37°C. Assay for the infection-associated antigen could be performed with heterologous antisera in view of the non-type specificity of this antigen. An assay for 12 S antigen in mixed antigen preparations could not be made. However, an assay with the isolated 12 S component indicated 0.039 µg/ml provided the end point using a fixation period of 20 hr at 4°C. The implications of the findings with respect to antibody titrations and diagnostic virus typing were discussed. The limitations of the complement-fixation technique as applied to foot and mouth disease were presented and the necessity of preparing specific antisera indicated." @default.
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- W4313376816 date "1967-10-01" @default.
- W4313376816 modified "2023-09-28" @default.
- W4313376816 title "Immunochemical Studies of Foot and Mouth Disease" @default.
- W4313376816 doi "https://doi.org/10.4049/jimmunol.99.4.729" @default.
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