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- W4313377032 abstract "Summary The primary and secondary immune responses of rabbits to HIFC were investigated with the radioimmunoelectrophoretic method. Three anti-HIFC-Co60B12-binding antibodies were detected. When a goat anti-rabbit serum was used as a developer, γM and γG antibodies were found to appear simultaneously, 7 to 8 days following primary immunization. When the developing serum was not used, an illusion suggesting the later appearance of γG antibody was seen. These results provide further evidence for the concurrent synthesis of γM and γG antibodies following primary immunization. In addition, the data suggest that early γG anti-HIF antibody forms a soluble or easily dissociable complex with HIFC-Co60B12 while later γG antibody forms an insoluble or less readily dissociable complex with the radioactively labeled antigen. In two of three rabbits, a third HIFC-Co60B12-binding antibody appeared from 3 to 4 weeks after primary immunization. This antibody migrated slightly faster than γG on electrophoresis in agar gel, was eluted with γG-globulins from Sephadex G-200, and was resistant to 2-mercaptoethanol. Following secondary immunization an anamnestic response was observed for the synthesis of all three antibodies." @default.
- W4313377032 created "2023-01-06" @default.
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- W4313377032 date "1967-03-01" @default.
- W4313377032 modified "2023-10-16" @default.
- W4313377032 title "Heterogeneity and Sequence of Appearance of Rabbit Antibodies to Hog Intrinsic Factor" @default.
- W4313377032 doi "https://doi.org/10.4049/jimmunol.98.3.558" @default.
- W4313377032 hasPublicationYear "1967" @default.
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