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- W4313379436 abstract "Abstract We have previously shown that RGC-32 is involved in cell cycle regulation in vitro. To define the in vivo role of RGC-32, we generated RGC-32 knockout mice. To assess the effect of RGC-32 deficiency on cell cycle activation in T cells, we determined the proliferative rates of CD4+ and CD8+ T cells from the spleens of RGC-32-/- mice, as compared to wild-type (WT) mice. After stimulation with anti-CD3/anti-CD28, CD4+ T cells from RGC-32-/- mice displayed a significant increase in [3H]-thymidine incorporation when compared to WT mice. In addition, both CD4+ and CD8+ T cells from RGC-32-/- mice displayed a significant increase in the proportion of proliferating Ki67+ cells, indicating that in T cells, RGC-32 has an inhibitory effect on cell cycle activation induced by T-cell receptor/CD28 engagement. Furthermore, Akt and FOXO1 phosphorylation induced in stimulated CD4+ T-cells from RGC-32-/- mice were significantly higher, indicating that RGC-32 inhibits cell cycle activation by suppressing FOXO1 activation. We also found that IL-2 mRNA and protein expression were significantly increased in RGC-32-/- CD4+ T cells when compared to WT CD4+ T cells. In addition, the effect of RGC-32 on the cell cycle and IL-2 expression was inhibited by pretreatment of the samples with LY294002, indicating a role for phosphatidylinositol 3- kinase (PI3K). Thus, RGC-32 is involved in controlling the cell cycle of T cells in vivo, and this effect is mediated by IL-2 in a PI3K-dependent fashion." @default.
- W4313379436 created "2023-01-06" @default.
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- W4313379436 date "2015-05-01" @default.
- W4313379436 modified "2023-09-27" @default.
- W4313379436 title "RGC-32 regulates T lymphocyte cell cycle activation in a phosphatidylinositol 3- kinase and IL-2 -dependent manner (IRC8P.442)" @default.
- W4313379436 doi "https://doi.org/10.4049/jimmunol.194.supp.129.6" @default.
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