FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313379835> ?p ?o ?g. }

• W4313379835 endingPage "463742" @default.
• W4313379835 startingPage "463742" @default.
• W4313379835 abstract "Reversed-phase peptide separation in hydrogen deuterium exchange (HDX) mass spectrometry (MS) must be done with conditions where the back exchange is the slowest possible, the so-called quench conditions of low pH and low temperature. To retain maximum deuterium, separation must also be done as quickly as possible. The low temperature (0 °C) of quench conditions complicates the separation and leads primarily to a reduction in separation quality and an increase in chromatographic backpressure. To improve the separation in HDX MS, one could use a longer gradient, smaller particles, a different separation mechanism (for example, capillary electrophoresis), or multi-dimensional separations such as combining ion mobility separation with reversed-phase separation. Another way to improve separations under HDX MS quench conditions is to use a higher flow rate where separation efficiency at 0 °C is more ideal. Higher flow rates, however, require chromatographic systems (both pumps and fittings) with higher backpressure limits. We tested what improvements could be realized with a commercial UPLC/UHPLC system capable of ∼20,000 psi backpressure. We found that a maximum flow rate of 225 µL/min (using a 1 × 50 mm column packed with 1.8 µm particles) was possible and that higher flow rate clearly led to higher peak capacity. HDX MS analysis of both simple and particularly complex samples improved, permitting both shorter separation time, if desired, and providing more deuterium recovery." @default.
• W4313379835 created "2023-01-06" @default.
• W4313379835 creator A5007768103 @default.
• W4313379835 creator A5010048926 @default.
• W4313379835 creator A5071831434 @default.
• W4313379835 creator A5082763070 @default.
• W4313379835 date "2023-01-01" @default.
• W4313379835 modified "2023-09-30" @default.
• W4313379835 title "Increase the flow rate and improve hydrogen deuterium exchange mass spectrometry" @default.
• W4313379835 cites W1976504585 @default.
• W4313379835 cites W2001098699 @default.
• W4313379835 cites W2007867267 @default.
• W4313379835 cites W2013029357 @default.
• W4313379835 cites W2029872563 @default.
• W4313379835 cites W2038572621 @default.
• W4313379835 cites W2039780381 @default.
• W4313379835 cites W2050413473 @default.
• W4313379835 cites W2052826095 @default.
• W4313379835 cites W2057642130 @default.
• W4313379835 cites W2058932040 @default.
• W4313379835 cites W2064485287 @default.
• W4313379835 cites W2069874231 @default.
• W4313379835 cites W2075613487 @default.
• W4313379835 cites W2082725885 @default.
• W4313379835 cites W2085801589 @default.
• W4313379835 cites W2108086364 @default.
• W4313379835 cites W2130079299 @default.
• W4313379835 cites W2131918208 @default.
• W4313379835 cites W2147024375 @default.
• W4313379835 cites W2148417867 @default.
• W4313379835 cites W2312984091 @default.
• W4313379835 cites W2321526934 @default.
• W4313379835 cites W2474650212 @default.
• W4313379835 cites W2507568318 @default.
• W4313379835 cites W2621276049 @default.
• W4313379835 cites W2792699653 @default.
• W4313379835 cites W281344288 @default.
• W4313379835 cites W2969325821 @default.
• W4313379835 cites W3009644416 @default.
• W4313379835 cites W3031963365 @default.
• W4313379835 cites W3094959102 @default.
• W4313379835 cites W3110644367 @default.
• W4313379835 cites W3138349348 @default.
• W4313379835 cites W3197273087 @default.
• W4313379835 cites W4284709305 @default.
• W4313379835 doi "https://doi.org/10.1016/j.chroma.2022.463742" @default.
• W4313379835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36586285" @default.
• W4313379835 hasPublicationYear "2023" @default.
• W4313379835 type Work @default.
• W4313379835 citedByCount "2" @default.
• W4313379835 countsByYear W43133798352022 @default.
• W4313379835 countsByYear W43133798352023 @default.
• W4313379835 crossrefType "journal-article" @default.
• W4313379835 hasAuthorship W4313379835A5007768103 @default.
• W4313379835 hasAuthorship W4313379835A5010048926 @default.
• W4313379835 hasAuthorship W4313379835A5071831434 @default.
• W4313379835 hasAuthorship W4313379835A5082763070 @default.
• W4313379835 hasBestOaLocation W43133798352 @default.
• W4313379835 hasConcept C113196181 @default.
• W4313379835 hasConcept C119857082 @default.
• W4313379835 hasConcept C121332964 @default.
• W4313379835 hasConcept C162356407 @default.
• W4313379835 hasConcept C167569220 @default.
• W4313379835 hasConcept C172120300 @default.
• W4313379835 hasConcept C178790620 @default.
• W4313379835 hasConcept C179998833 @default.
• W4313379835 hasConcept C184779094 @default.
• W4313379835 hasConcept C185592680 @default.
• W4313379835 hasConcept C2776061190 @default.
• W4313379835 hasConcept C2992520659 @default.
• W4313379835 hasConcept C41008148 @default.
• W4313379835 hasConcept C43617362 @default.
• W4313379835 hasConcept C512968161 @default.
• W4313379835 hasConcept C58364064 @default.
• W4313379835 hasConcept C97355855 @default.
• W4313379835 hasConceptScore W4313379835C113196181 @default.
• W4313379835 hasConceptScore W4313379835C119857082 @default.
• W4313379835 hasConceptScore W4313379835C121332964 @default.
• W4313379835 hasConceptScore W4313379835C162356407 @default.
• W4313379835 hasConceptScore W4313379835C167569220 @default.
• W4313379835 hasConceptScore W4313379835C172120300 @default.
• W4313379835 hasConceptScore W4313379835C178790620 @default.
• W4313379835 hasConceptScore W4313379835C179998833 @default.
• W4313379835 hasConceptScore W4313379835C184779094 @default.
• W4313379835 hasConceptScore W4313379835C185592680 @default.
• W4313379835 hasConceptScore W4313379835C2776061190 @default.
• W4313379835 hasConceptScore W4313379835C2992520659 @default.
• W4313379835 hasConceptScore W4313379835C41008148 @default.
• W4313379835 hasConceptScore W4313379835C43617362 @default.
• W4313379835 hasConceptScore W4313379835C512968161 @default.
• W4313379835 hasConceptScore W4313379835C58364064 @default.
• W4313379835 hasConceptScore W4313379835C97355855 @default.
• W4313379835 hasLocation W43133798351 @default.
• W4313379835 hasLocation W43133798352 @default.
• W4313379835 hasLocation W43133798353 @default.
• W4313379835 hasOpenAccess W4313379835 @default.
• W4313379835 hasPrimaryLocation W43133798351 @default.
• W4313379835 hasRelatedWork W1580673954 @default.

• next