FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313381237> ?p ?o ?g. }

• W4313381237 abstract "Conventional immunohistochemistry technologies were limited by the inability to simultaneously detect multiple markers and the lack of identifying spatial relationships among cells, hindering understanding of the biological processes in cancer immunology.Tissue slices of primary tumours from 553 IA∼IIIB non-small cell lung cancer (NSCLC) cases were stained by multiplex immunofluorescence (mIF) assay for 10 markers, including CD4, CD38, CD20, FOXP3, CD66b, CD8, CD68, PD-L1, CD133 and CD163, evaluating the amounts of 26 phenotypes of cells in tumour nest and tumour stroma. StarDist depth learning model was utilised to determine the spatial location of cells based on mIF graphs. Single-cell RNA sequencing (scRNA-seq) on four primary NSCLC cases was conducted to investigate the putative cell interaction networks.Spatial proximity among CD20+ B cells, CD4+ T cells and CD38+ T cells (r2 = 0.41) was observed, whereas the distribution of regulatory T cells was associated with decreased infiltration levels of CD20+ B cells and CD38+ T cells (r2 = -0.45). Univariate Cox analyses identified closer proximity between CD8+ T cells predicted longer disease-free survival (DFS). In contrast, closer proximity between CD133+ cancer stem cells (CSCs), longer distances between CD4+ T cells and CD20+ B cells, CD4+ T cells and neutrophils, and CD20+ B cells and neutrophils were correlated with dismal DFS. Data from scRNA-seq further showed that spatially adjacent N1-like neutrophils could boost the proliferation and activation of T and B lymphocytes, whereas spatially neighbouring M2-like macrophages showed negative effects. An immune-related risk score (IRRS) system aggregating robust quantitative and spatial prognosticators showed that high-IRRS patients had significantly worse DFS than low-IRRS ones (HR 2.72, 95% CI 1.87-3.94, p < .001).We developed a framework to analyse the cell interaction networks in tumour microenvironment, revealing the spatial architecture and intricate interplays between immune and tumour cells." @default.
• W4313381237 created "2023-01-06" @default.
• W4313381237 creator A5008749330 @default.
• W4313381237 creator A5008910146 @default.
• W4313381237 creator A5014237587 @default.
• W4313381237 creator A5018628399 @default.
• W4313381237 creator A5027835055 @default.
• W4313381237 creator A5031255136 @default.
• W4313381237 creator A5031664319 @default.
• W4313381237 creator A5033215610 @default.
• W4313381237 creator A5047774700 @default.
• W4313381237 creator A5064055416 @default.
• W4313381237 creator A5074447759 @default.
• W4313381237 creator A5076115952 @default.
• W4313381237 creator A5086842889 @default.
• W4313381237 creator A5091520888 @default.
• W4313381237 date "2023-01-01" @default.
• W4313381237 modified "2023-10-09" @default.
• W4313381237 title "Multiplex immunofluorescence and single‐cell transcriptomic profiling reveal the spatial cell interaction networks in the non‐small cell lung cancer microenvironment" @default.
• W4313381237 cites W1569332748 @default.
• W4313381237 cites W1595547013 @default.
• W4313381237 cites W1969057487 @default.
• W4313381237 cites W2002157461 @default.
• W4313381237 cites W2021733365 @default.
• W4313381237 cites W2035252444 @default.
• W4313381237 cites W2066968134 @default.
• W4313381237 cites W2067931754 @default.
• W4313381237 cites W2071123870 @default.
• W4313381237 cites W2099900951 @default.
• W4313381237 cites W2130410032 @default.
• W4313381237 cites W2144408907 @default.
• W4313381237 cites W2146512944 @default.
• W4313381237 cites W2170164215 @default.
• W4313381237 cites W2507880739 @default.
• W4313381237 cites W2564088321 @default.
• W4313381237 cites W2760946358 @default.
• W4313381237 cites W2787894829 @default.
• W4313381237 cites W2796207838 @default.
• W4313381237 cites W2796558260 @default.
• W4313381237 cites W2801309958 @default.
• W4313381237 cites W2809205318 @default.
• W4313381237 cites W2811247652 @default.
• W4313381237 cites W2865352686 @default.
• W4313381237 cites W2890156321 @default.
• W4313381237 cites W2900411771 @default.
• W4313381237 cites W2910849865 @default.
• W4313381237 cites W2914335731 @default.
• W4313381237 cites W2922475342 @default.
• W4313381237 cites W2923394609 @default.
• W4313381237 cites W2936414047 @default.
• W4313381237 cites W2937848504 @default.
• W4313381237 cites W2963398239 @default.
• W4313381237 cites W2971041600 @default.
• W4313381237 cites W2973186268 @default.
• W4313381237 cites W2990860490 @default.
• W4313381237 cites W2994223194 @default.
• W4313381237 cites W2998425601 @default.
• W4313381237 cites W2998794254 @default.
• W4313381237 cites W3004765765 @default.
• W4313381237 cites W3012413426 @default.
• W4313381237 cites W3016553397 @default.
• W4313381237 cites W3024300243 @default.
• W4313381237 cites W3024919611 @default.
• W4313381237 cites W3047081808 @default.
• W4313381237 cites W3084976682 @default.
• W4313381237 cites W3100296314 @default.
• W4313381237 cites W3111471842 @default.
• W4313381237 cites W3118438602 @default.
• W4313381237 cites W3119618910 @default.
• W4313381237 cites W3126902084 @default.
• W4313381237 cites W3128646645 @default.
• W4313381237 cites W3132661792 @default.
• W4313381237 cites W3150581040 @default.
• W4313381237 cites W3157423185 @default.
• W4313381237 cites W3172039897 @default.
• W4313381237 cites W3172599325 @default.
• W4313381237 cites W3175752499 @default.
• W4313381237 cites W3185971835 @default.
• W4313381237 cites W3189383967 @default.
• W4313381237 cites W3202362978 @default.
• W4313381237 cites W3210191307 @default.
• W4313381237 cites W3213622352 @default.
• W4313381237 cites W3214233691 @default.
• W4313381237 cites W4200498032 @default.
• W4313381237 cites W4205925879 @default.
• W4313381237 cites W4224245311 @default.
• W4313381237 cites W4281564308 @default.
• W4313381237 cites W4292482497 @default.
• W4313381237 cites W4292528167 @default.
• W4313381237 cites W4293237390 @default.
• W4313381237 cites W4308620781 @default.
• W4313381237 cites W4308834549 @default.
• W4313381237 cites W4313381237 @default.
• W4313381237 doi "https://doi.org/10.1002/ctm2.1155" @default.
• W4313381237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36588094" @default.
• W4313381237 hasPublicationYear "2023" @default.
• W4313381237 type Work @default.
• W4313381237 citedByCount "7" @default.
• W4313381237 countsByYear W43133812372023 @default.
• W4313381237 crossrefType "journal-article" @default.

• next