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- W4313381502 abstract "Abstract Neutrophils and monocytes have previously been shown to be important for host protection during infection with Listeria monocytogenes (Lm). Previous studies have shown that simultaneous depletion of neutrophils and monocytes with the Gr-1 antibody leads to susceptibility to Lm infection. The purpose of these studies is to delineate differences in function between neutrophils and monocytes during Lm infection. Neutrophils obtained from the bone marrow, liver and spleen of C57BL/6 mice were more effective at phagocytosis of Lm in comparison to monocytes as they had a higher total bacteria MFI than the monocytes. To determine differences in the ability of the cells to keep bacteria contained in the phagosome, the cells were infected with a strain of Lm that only expresses GFP when the bacteria escapes out of the phagosome into the cytosol. Comparison of the MFI of total bacteria present vs escaped bacteria showed that monocytes allowed for more bacteria to escape in comparison to neutrophils. Therefore, monocytes are less effective at bacterial containment in comparison to neutrophils. To ascertain differences in killing ability, bone marrow neutrophils and monocytes were sorted for a killing assay and neutrophils were also more effective than monocytes at bacterial killing. In conclusion, although both cell types are important for protection during Lm infection, neutrophils appear to be essential for protection as they are more effective at phagocytosis, phagosomal containment and bacteria killing. Future studies, such as measurement of ROS/RNS as well as cytokine production, will aid in further defining specific functional differences between neutrophils and monocytes during intracellular bacterial infection." @default.
- W4313381502 created "2023-01-06" @default.
- W4313381502 creator A5064602936 @default.
- W4313381502 creator A5082582893 @default.
- W4313381502 date "2019-05-01" @default.
- W4313381502 modified "2023-09-23" @default.
- W4313381502 title "Neutrophils are more effective than monocytes at containment and killing of <i>Listeria monocytogenes</i>" @default.
- W4313381502 doi "https://doi.org/10.4049/jimmunol.202.supp.126.19" @default.
- W4313381502 hasPublicationYear "2019" @default.
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