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- W4313381808 abstract "Abstract The CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) platform is a recent advance in single cell analysis, which is based on high-throughput single cell sequencing (scSeq) and combines measurements of cellular proteins and transcriptomes. This platform will potentially transform how complex cell populations (lineage differentiation or tumor infiltrating lymphocytes) are studied. Published data indicated that CITE-Seq analysis on cell surface marker expression are comparable to multi-color flow cytometry, but provides superior capacity in multiplexing to the latter. Currently, individual investigators use their choice of oligo bar-codes for different protein markers and there is no available control. This will make comparison of data from different studies difficult in the future. The availability of standardized oligonucleotide bar-code labeled antibodies as well as control cells will enable reliable comparison data from longitudinal studies or among different studies. FlexSeq™ is our new product line, in which monoclonal antibodies are conjugated with unique oligonucleotide bar-codes. We provide standardized bar-coding system and ready to use oligo-antibody conjugates to support CITE-Seq based multiplex immunophenotyping. Veri-Cells™ is our proprietary lyophilized cell product that can be used as a control for detection of cell surface and intracellular flow cytometry. In this study, we validate the application of FlexSeq™ products in CITE-Seq platform, and compare Veri-Cells™ PBMC with freshly isolated PBMC in the assays." @default.
- W4313381808 created "2023-01-06" @default.
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- W4313381808 date "2018-05-01" @default.
- W4313381808 modified "2023-09-27" @default.
- W4313381808 title "Standardized oligo barcode antibody conjugates, and Veri-Cells™ PBMC for multiplex immunophenotyping and CITE-Seq" @default.
- W4313381808 doi "https://doi.org/10.4049/jimmunol.200.supp.174.19" @default.
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