Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313381888> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4313381888 endingPage "176.10" @default.
- W4313381888 startingPage "176.10" @default.
- W4313381888 abstract "Abstract Low-dose IL-2 therapy is a direct approach to boost Tregs and promote immune tolerance in autoimmune patients. The selective response of Tregs to low-dose IL-2 has been suggested to be due to high level of CD25 and activity of the Ser/Thr phosphatase, PP2A. Here we directly assessed the contribution of these two parameters in promoting IL-2 signaling in human Tregs. IL-2-induced tyrosine phosphorylation of STAT5 (pSTAT5) was proportional to CD25 levels on human CD4+ T cells, directly demonstrating that high IL-2R levels promote the Treg response to low-dose IL-2. To evaluate the role of PP2A, expanded human Tregs were transduced with lentivirus to overexpress or knock down the PP2A catalytic subunit (PP2Ac). Overexpression of PP2Ac increased the level of all IL-2R subunits and promoted phosphorylation of Jak3 at Tyr980/981. Compared to control-transduced cells, PP2Ac-overexpressed Tregs were 3-fold more sensitive to IL-2 based on pSTAT5 activation. Increased expression of CD25 only partially accounted for this enhanced activation of pSTAT5. Consistent with these findings, knockdown of PP2Ac in Tregs significantly reduced the pSTAT5 responses to IL-2. Overexpression of PP2Ac in Tregs also increased the expressions of proteins related to growth, survival, activation, and immunosuppressive function, and included several IL-2-regulated genes. Collectively, these findings indicate that PP2A promotes IL-2R signaling through multiple mechanisms, one related to control of IL-2R expression and another that may directly regulate IL-2R signaling." @default.
- W4313381888 created "2023-01-06" @default.
- W4313381888 creator A5065361211 @default.
- W4313381888 creator A5071530964 @default.
- W4313381888 creator A5072424476 @default.
- W4313381888 date "2018-05-01" @default.
- W4313381888 modified "2023-09-23" @default.
- W4313381888 title "Protein phosphatase 2A promotes IL-2R signaling in human regulatory T cells" @default.
- W4313381888 doi "https://doi.org/10.4049/jimmunol.200.supp.176.10" @default.
- W4313381888 hasPublicationYear "2018" @default.
- W4313381888 type Work @default.
- W4313381888 citedByCount "0" @default.
- W4313381888 crossrefType "journal-article" @default.
- W4313381888 hasAuthorship W4313381888A5065361211 @default.
- W4313381888 hasAuthorship W4313381888A5071530964 @default.
- W4313381888 hasAuthorship W4313381888A5072424476 @default.
- W4313381888 hasConcept C104292427 @default.
- W4313381888 hasConcept C104317684 @default.
- W4313381888 hasConcept C11960822 @default.
- W4313381888 hasConcept C173396325 @default.
- W4313381888 hasConcept C178666793 @default.
- W4313381888 hasConcept C185592680 @default.
- W4313381888 hasConcept C203014093 @default.
- W4313381888 hasConcept C2776090121 @default.
- W4313381888 hasConcept C2777553839 @default.
- W4313381888 hasConcept C2779391668 @default.
- W4313381888 hasConcept C389152 @default.
- W4313381888 hasConcept C502942594 @default.
- W4313381888 hasConcept C55493867 @default.
- W4313381888 hasConcept C62478195 @default.
- W4313381888 hasConcept C79484868 @default.
- W4313381888 hasConcept C86803240 @default.
- W4313381888 hasConcept C8891405 @default.
- W4313381888 hasConcept C95444343 @default.
- W4313381888 hasConceptScore W4313381888C104292427 @default.
- W4313381888 hasConceptScore W4313381888C104317684 @default.
- W4313381888 hasConceptScore W4313381888C11960822 @default.
- W4313381888 hasConceptScore W4313381888C173396325 @default.
- W4313381888 hasConceptScore W4313381888C178666793 @default.
- W4313381888 hasConceptScore W4313381888C185592680 @default.
- W4313381888 hasConceptScore W4313381888C203014093 @default.
- W4313381888 hasConceptScore W4313381888C2776090121 @default.
- W4313381888 hasConceptScore W4313381888C2777553839 @default.
- W4313381888 hasConceptScore W4313381888C2779391668 @default.
- W4313381888 hasConceptScore W4313381888C389152 @default.
- W4313381888 hasConceptScore W4313381888C502942594 @default.
- W4313381888 hasConceptScore W4313381888C55493867 @default.
- W4313381888 hasConceptScore W4313381888C62478195 @default.
- W4313381888 hasConceptScore W4313381888C79484868 @default.
- W4313381888 hasConceptScore W4313381888C86803240 @default.
- W4313381888 hasConceptScore W4313381888C8891405 @default.
- W4313381888 hasConceptScore W4313381888C95444343 @default.
- W4313381888 hasIssue "1_Supplement" @default.
- W4313381888 hasLocation W43133818881 @default.
- W4313381888 hasOpenAccess W4313381888 @default.
- W4313381888 hasPrimaryLocation W43133818881 @default.
- W4313381888 hasRelatedWork W1995690959 @default.
- W4313381888 hasRelatedWork W1998662277 @default.
- W4313381888 hasRelatedWork W2033875669 @default.
- W4313381888 hasRelatedWork W2046487040 @default.
- W4313381888 hasRelatedWork W2078377364 @default.
- W4313381888 hasRelatedWork W2081280187 @default.
- W4313381888 hasRelatedWork W2084352760 @default.
- W4313381888 hasRelatedWork W2091016107 @default.
- W4313381888 hasRelatedWork W2113269934 @default.
- W4313381888 hasRelatedWork W2149203347 @default.
- W4313381888 hasVolume "200" @default.
- W4313381888 isParatext "false" @default.
- W4313381888 isRetracted "false" @default.
- W4313381888 workType "article" @default.