Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313382145> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W4313382145 endingPage "147.12" @default.
- W4313382145 startingPage "147.12" @default.
- W4313382145 abstract "Abstract No vaccine exists against visceral leishmaniasis. Towards developing an effective vaccine, we have reported extensively on the immunogenicity of live attenuated LdCentrin−/− mutants in animal models. However, for the use of LdCen1−/− in humans there is a need to develop biomarkers that can predict the immunogenicity of LdCen−/− at an earlier stage of vaccination. Unbiased gene expression profiling of US blood donor’s PBMCs infected with Leishmania parasites (LdWT or LdCen−/−) ex-vivo resulted in the identification of several miRs that regulate important cytokine genes. Importantly, we found a strong induction of miR-21, known to control the expression of IL-12 mRNA, in LdWT infection compared to LdCen−/− infection. IL-12 produced by DCs is critical for priming a host protective Th1 cell response during Leishmania infection. Validation of human PBMC data in a mouse model revealed an enhanced expression of miR-21 in virulent LdWT infected dendritic cells (DCs) compared to LdCen−/−. This correlated with low levels of IL-12 mRNA in WT compared to LdCen−/− infected DCs. Silencing of miR-21 resulted in an augmented induction of IL12 in LdWT infected DCs both in vitro and in vivo, illustrating the role of miR-21 in LdWT infection mediated suppression of IL12. In contrast, LdCen−/− infected DCs showed a strong induction of IL12, and miR-21 silencing resulted in a further increase in IL12 levels. Additionally, blocking of miR-21 enabled strong proliferation of CD4+ T cells in mice infected with LdWT parasites. Thus lower level of miR-21 in a vaccinee may indicate the safety and immunogenicity consistent with a protective response of the vaccine. Our data suggests that miR-21 could be used as a biomarker of protection in anti-leishmanial vaccines." @default.
- W4313382145 created "2023-01-06" @default.
- W4313382145 creator A5000479943 @default.
- W4313382145 creator A5059468034 @default.
- W4313382145 creator A5077207677 @default.
- W4313382145 creator A5088484714 @default.
- W4313382145 creator A5089647417 @default.
- W4313382145 date "2017-05-01" @default.
- W4313382145 modified "2023-09-24" @default.
- W4313382145 title "Identification of microRNA-21 as a biomarker in live attenuated Leishmania vaccine induced protective immunity" @default.
- W4313382145 doi "https://doi.org/10.4049/jimmunol.198.supp.147.12" @default.
- W4313382145 hasPublicationYear "2017" @default.
- W4313382145 type Work @default.
- W4313382145 citedByCount "1" @default.
- W4313382145 countsByYear W43133821452021 @default.
- W4313382145 crossrefType "journal-article" @default.
- W4313382145 hasAuthorship W4313382145A5000479943 @default.
- W4313382145 hasAuthorship W4313382145A5059468034 @default.
- W4313382145 hasAuthorship W4313382145A5077207677 @default.
- W4313382145 hasAuthorship W4313382145A5088484714 @default.
- W4313382145 hasAuthorship W4313382145A5089647417 @default.
- W4313382145 hasConcept C100701293 @default.
- W4313382145 hasConcept C104317684 @default.
- W4313382145 hasConcept C119056186 @default.
- W4313382145 hasConcept C136764020 @default.
- W4313382145 hasConcept C137061746 @default.
- W4313382145 hasConcept C149532602 @default.
- W4313382145 hasConcept C150903083 @default.
- W4313382145 hasConcept C154317977 @default.
- W4313382145 hasConcept C159047783 @default.
- W4313382145 hasConcept C202751555 @default.
- W4313382145 hasConcept C203014093 @default.
- W4313382145 hasConcept C207001950 @default.
- W4313382145 hasConcept C26291073 @default.
- W4313382145 hasConcept C2778170410 @default.
- W4313382145 hasConcept C2778690821 @default.
- W4313382145 hasConcept C2780599195 @default.
- W4313382145 hasConcept C2780868878 @default.
- W4313382145 hasConcept C2781092759 @default.
- W4313382145 hasConcept C41008148 @default.
- W4313382145 hasConcept C55493867 @default.
- W4313382145 hasConcept C59822182 @default.
- W4313382145 hasConcept C71928629 @default.
- W4313382145 hasConcept C81444415 @default.
- W4313382145 hasConcept C86803240 @default.
- W4313382145 hasConcept C8891405 @default.
- W4313382145 hasConceptScore W4313382145C100701293 @default.
- W4313382145 hasConceptScore W4313382145C104317684 @default.
- W4313382145 hasConceptScore W4313382145C119056186 @default.
- W4313382145 hasConceptScore W4313382145C136764020 @default.
- W4313382145 hasConceptScore W4313382145C137061746 @default.
- W4313382145 hasConceptScore W4313382145C149532602 @default.
- W4313382145 hasConceptScore W4313382145C150903083 @default.
- W4313382145 hasConceptScore W4313382145C154317977 @default.
- W4313382145 hasConceptScore W4313382145C159047783 @default.
- W4313382145 hasConceptScore W4313382145C202751555 @default.
- W4313382145 hasConceptScore W4313382145C203014093 @default.
- W4313382145 hasConceptScore W4313382145C207001950 @default.
- W4313382145 hasConceptScore W4313382145C26291073 @default.
- W4313382145 hasConceptScore W4313382145C2778170410 @default.
- W4313382145 hasConceptScore W4313382145C2778690821 @default.
- W4313382145 hasConceptScore W4313382145C2780599195 @default.
- W4313382145 hasConceptScore W4313382145C2780868878 @default.
- W4313382145 hasConceptScore W4313382145C2781092759 @default.
- W4313382145 hasConceptScore W4313382145C41008148 @default.
- W4313382145 hasConceptScore W4313382145C55493867 @default.
- W4313382145 hasConceptScore W4313382145C59822182 @default.
- W4313382145 hasConceptScore W4313382145C71928629 @default.
- W4313382145 hasConceptScore W4313382145C81444415 @default.
- W4313382145 hasConceptScore W4313382145C86803240 @default.
- W4313382145 hasConceptScore W4313382145C8891405 @default.
- W4313382145 hasIssue "1_Supplement" @default.
- W4313382145 hasLocation W43133821451 @default.
- W4313382145 hasOpenAccess W4313382145 @default.
- W4313382145 hasPrimaryLocation W43133821451 @default.
- W4313382145 hasRelatedWork W2009685269 @default.
- W4313382145 hasRelatedWork W2017012825 @default.
- W4313382145 hasRelatedWork W2056046212 @default.
- W4313382145 hasRelatedWork W2087976276 @default.
- W4313382145 hasRelatedWork W2142624029 @default.
- W4313382145 hasRelatedWork W2161870666 @default.
- W4313382145 hasRelatedWork W2165829182 @default.
- W4313382145 hasRelatedWork W2252537997 @default.
- W4313382145 hasRelatedWork W2608261238 @default.
- W4313382145 hasRelatedWork W4313374704 @default.
- W4313382145 hasVolume "198" @default.
- W4313382145 isParatext "false" @default.
- W4313382145 isRetracted "false" @default.
- W4313382145 workType "article" @default.