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- W4313383798 abstract "Abstract Interleukin-4 is an important Th2 type cytokine in mammals which regulates growth and differentiation of B Lymphocytes. On the mammalian counterparts, the function of IL-4 orthologue in chickens (chIL-4) is less understood. Although the source of chIL-4 is not well established, it has been shown to have an integral role in mediating Th2 type immune responses in chickens. Despite its significant role, no monoclonal antibodies (mAbs) against chIL-4 have been developed till date. Therefore, in the present study we used recombinant yeast-expressed chIL-4 as an immunogen to develop mAbs against chIL-4 to establish the basis for understanding the function of chIL-4 in regulating Th2 mediated immune responses in chickens. We developed two mAbs against chIL-4 and characterized their specificity by western blot, enzyme linked immunosorbent assay (ELISA) and intracellular staining (ICS). These antibodies displayed specific binding with the recombinant chl-IL-4 as well as endogenous chIL-4 by western blot, ELISA and ICS. Further, these antibodies neutralized the inhibitory effect of chIL-4 on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and expression of inducible nitric oxide synthase (iNOS) in avian macrophage-like HD11 cells, thus confirming that these antibodies can block the chIL-4 bioactivity. In conclusion, the mAbs developed in the present study can be useful for understanding the role of chIL-4 in the basic concept of Th1 versus Th2 T cell subsets in chickens." @default.
- W4313383798 created "2023-01-06" @default.
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- W4313383798 date "2018-05-01" @default.
- W4313383798 modified "2023-09-23" @default.
- W4313383798 title "Production and characterization of monoclonal antibodies specific for chicken interleukin-4" @default.
- W4313383798 doi "https://doi.org/10.4049/jimmunol.200.supp.164.22" @default.
- W4313383798 hasPublicationYear "2018" @default.
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