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- W4313384292 abstract "Abstract Accumulating evidence reveals that changes in the epigenome regulate gene expression profiles of developing, differentiating, and effector immune cells. Importantly, disturbances in this developmental road map may contribute to aberrant immune responses. The recent development of the Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) allows for the epigenetic programming of rare immune cells to be determined. This method utilizes the Tn5 transposase to simultaneously fragment and tag genomic DNA by inserting Illumina sequencing adapters. Unique sequencing indexes are then added to the adapter-modified genomic fragments during the PCR enrichment step of sample preparation. This tagmentation-based approach has emerged as a popular approach to generating sequence-ready libraries. Here, we describe a novel strategy in which we have customized the tagmentation process by using in-house purified Tn5 transposase pre-loaded with indexed adapters. This method allows for a more efficient analysis of samples which increases experimental throughput while significantly reducing sequencing cost." @default.
- W4313384292 created "2023-01-06" @default.
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- W4313384292 date "2017-05-01" @default.
- W4313384292 modified "2023-09-23" @default.
- W4313384292 title "TULIPES: Tn5-mediated ultra low input parallel epigenetic sequencing" @default.
- W4313384292 doi "https://doi.org/10.4049/jimmunol.198.supp.81.19" @default.
- W4313384292 hasPublicationYear "2017" @default.
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