Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313384377> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W4313384377 endingPage "81.9" @default.
- W4313384377 startingPage "81.9" @default.
- W4313384377 abstract "Abstract Platelets are non-nucleated cellular fragments circulating in the peripheral blood, derived from megakaryocytes. They are critical in the maintenance of hemostasis. Alterations in their function are associated with a range of clinical conditions. Using flow cytometry, light scatter signals from platelets typically overlap signals from red blood cells (RBC). Since RBC outnumber platelets by more than an order of magnitude in whole blood, platelets are typically studied by depleting samples of RBCs by centrifugation methods or selective RBC cell lysis. These sample preparation procedures require manipulations that may affect platelet health and function, and result in loss of cells. Additionally, lysed samples require labeling of the platelets with a fluorescent marker to adequately resolve the platelets from debris/noise. We present a novel technique for resolving leukocytes and platelets from RBC in whole blood using orthogonal light scatter from 2 lasers. This method exploits the differences in light-scattering properties between RBCs and platelets and white blood cells (WBC). Hemoglobin readily absorbs 405 nm laser light, reducing the scatter signal from RBC relative to WBCs and platelets, resulting in a reproducible scatter pattern when analyzing human whole blood with both blue (488 nm) and violet (405 nm) side scatter. Using this approach, a distinct platelet population can be discerned in unaltered whole blood, requiring only dilution of whole blood in buffer. Combining this approach with acoustic cytometry allows great flexibility in determining optimal dilution and sample collection rates for minimal coincidence and rapid analysis. For research use only. Not for use in diagnostic procedures." @default.
- W4313384377 created "2023-01-06" @default.
- W4313384377 creator A5005678901 @default.
- W4313384377 creator A5033813445 @default.
- W4313384377 date "2017-05-01" @default.
- W4313384377 modified "2023-09-25" @default.
- W4313384377 title "Platelet Detection in Unaltered Whole Blood" @default.
- W4313384377 doi "https://doi.org/10.4049/jimmunol.198.supp.81.9" @default.
- W4313384377 hasPublicationYear "2017" @default.
- W4313384377 type Work @default.
- W4313384377 citedByCount "0" @default.
- W4313384377 crossrefType "journal-article" @default.
- W4313384377 hasAuthorship W4313384377A5005678901 @default.
- W4313384377 hasAuthorship W4313384377A5033813445 @default.
- W4313384377 hasConcept C126322002 @default.
- W4313384377 hasConcept C1621761 @default.
- W4313384377 hasConcept C185592680 @default.
- W4313384377 hasConcept C203014093 @default.
- W4313384377 hasConcept C2778589496 @default.
- W4313384377 hasConcept C2780339063 @default.
- W4313384377 hasConcept C2908647359 @default.
- W4313384377 hasConcept C553184892 @default.
- W4313384377 hasConcept C55493867 @default.
- W4313384377 hasConcept C57409179 @default.
- W4313384377 hasConcept C71924100 @default.
- W4313384377 hasConcept C86803240 @default.
- W4313384377 hasConcept C89560881 @default.
- W4313384377 hasConcept C99454951 @default.
- W4313384377 hasConceptScore W4313384377C126322002 @default.
- W4313384377 hasConceptScore W4313384377C1621761 @default.
- W4313384377 hasConceptScore W4313384377C185592680 @default.
- W4313384377 hasConceptScore W4313384377C203014093 @default.
- W4313384377 hasConceptScore W4313384377C2778589496 @default.
- W4313384377 hasConceptScore W4313384377C2780339063 @default.
- W4313384377 hasConceptScore W4313384377C2908647359 @default.
- W4313384377 hasConceptScore W4313384377C553184892 @default.
- W4313384377 hasConceptScore W4313384377C55493867 @default.
- W4313384377 hasConceptScore W4313384377C57409179 @default.
- W4313384377 hasConceptScore W4313384377C71924100 @default.
- W4313384377 hasConceptScore W4313384377C86803240 @default.
- W4313384377 hasConceptScore W4313384377C89560881 @default.
- W4313384377 hasConceptScore W4313384377C99454951 @default.
- W4313384377 hasIssue "1_Supplement" @default.
- W4313384377 hasLocation W43133843771 @default.
- W4313384377 hasOpenAccess W4313384377 @default.
- W4313384377 hasPrimaryLocation W43133843771 @default.
- W4313384377 hasRelatedWork W1968985065 @default.
- W4313384377 hasRelatedWork W2040403406 @default.
- W4313384377 hasRelatedWork W2042295537 @default.
- W4313384377 hasRelatedWork W2067724718 @default.
- W4313384377 hasRelatedWork W2129894791 @default.
- W4313384377 hasRelatedWork W2131567659 @default.
- W4313384377 hasRelatedWork W2354275169 @default.
- W4313384377 hasRelatedWork W2744274866 @default.
- W4313384377 hasRelatedWork W2906142706 @default.
- W4313384377 hasRelatedWork W3008026583 @default.
- W4313384377 hasVolume "198" @default.
- W4313384377 isParatext "false" @default.
- W4313384377 isRetracted "false" @default.
- W4313384377 workType "article" @default.